Viability of freshly prepared cells derived from foetal brain of rabbit at different temperature settings during transportation

Abdul Razak, Redzuan Nul Hakim and Mazlan, Muhammad Alif and Ahmad, Azmir and Abd. Jalil, Mohd. Azri and Abdul Jalil, Abdul Halim and Md Isa, Muhammad Lokman (2020) Viability of freshly prepared cells derived from foetal brain of rabbit at different temperature settings during transportation. Malaysian Journal of Biochemistry and Molecular Biology, 23 (3). pp. 88-97. ISSN 1511- 2616 E-ISSN 2600-9005

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Abstract

The characteristics of rabbit cells, i.e., high proliferative activity, high passage numbers, and morphologically similar to human cells renders it a potential source of a cell line. Nonetheless, they are not widely characterised for research purposes. This study aimed to observe the effects of different temperature settings on the viability of rabbit foetal brain (RFB) cells at different time points. The harvested RFB cells were divided into room temperature (RT), ice packs (ICE), and refrigerator (4 °C) groups. Cell viability was determined at 0, 6, 12, 24, 48, and 72 h. Following cell viability determination, the cells were recultured for 24 h to observe cell viability and morphology post-transportation. The cell viability was analysed statistically for within and more than 24 h duration using the one-way ANOVA test, and the cell morphology was observed under the microscope. For the within 24 h analysis, the ICE group showed the best condition for 24 h transportation due to significantly higher cell viability (p<0.05) compared to the RT group. Meanwhile, for the more than 24 h analysis, the RT group showed the best condition for more than 24 h transportation due to higher cell viability compared to the ICE and 4 °C groups, albeit the non-significance. The reculturing of RFB cells showed no remarkable difference in the cell viability and morphology in all transportation time points at all temperature settings. In conclusion, the study found that the RFB cells are suitable to be transported using ice packs for transportation within 24 h and room temperature for transportation of more than 24 h.

Item Type:	Article (Journal)
Uncontrolled Keywords:	Rabbit foetal brain cells; Cell viability; Temperature; Transportation time
Subjects:	Q Science > QP Physiology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Nursing Kulliyyah of Nursing > Department of Basic Medical Sciences for Nursing
Depositing User:	Redzuan Nul Hakim Abdul Razak
Date Deposited:	31 May 2021 15:41
Last Modified:	31 May 2021 15:41
URI:	http://irep.iium.edu.my/id/eprint/90018

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