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Naked Eye Colorimetric Glucose Detection Using Microplate Reader

Azahan, Siti Nurul Fatihah and Mohd Nasir, Mohd Hamzah and Mohd Salleh, Mohd Hazimin (2021) Naked Eye Colorimetric Glucose Detection Using Microplate Reader. Journal of Physics: Conference Series, 1892 (012020). pp. 1-5. ISSN 1742-6588 E-ISSN 1742-6596

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Diabetes in general can be classified as Type 1, Type 2, gestational diabetes mellitus that occur during pregnancy, and specific types of diabetes due to certain health condition. Monitoring glucose level is crucial in order to maintain a healthy lifestyle, especially among diabetic patients. Today, researchers looking for a less painful, cheap, and user-friendly noninvasive method to monitor the body glucose level. One of the promising approaches is sweat based glucose detection. In order to calibrate the sensing device, we determined the limit of detection of the glucose concentration in sweat liquid by the well-established enzymatic reaction. This paper will discuss the utilisation of a microplate reader to measure the sensitivity of the chromogenic change in reaction. 50μL of glucose oxidase-peroxidase (GOD-POD) enzyme and 1.67μL glucose sample with different concentrations were mixed in 96 well flat bottom microplate, and the absorbance were detected by using a microplate reader. The absorbance value was used to calculate the concentration. The calculated concentration was compared with the known concentration. From the experimental procedure, the results showed the consistency of calculated and known concentration and R2 = 0.9995

Item Type: Article (Journal)
Additional Information: 89917/4794
Uncontrolled Keywords: enzymatic, glucose oxidase-peroxidase, sweat based glucose detection
Subjects: Q Science > QC Physics
Q Science > QD Chemistry
Q Science > QH Natural history > QH301 Biology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Science > Department of Biotechnology
Kulliyyah of Science > Department of Physics
Depositing User: Dr. Mohd. Hazimin Mohd. Salleh
Date Deposited: 20 May 2021 16:30
Last Modified: 20 May 2021 16:30
URI: http://irep.iium.edu.my/id/eprint/89917

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