Midin, Mohd Razik and Fikri, Muhammad Irfan and Zailani, Siti Sarah
(2020)
Nuclear genome size determination of Christia Vespertilionis via flow cytometry.
Genome Size Determination Of Christia Vespertilionis Via Flow Cytometry, 4 (2).
pp. 72-75.
ISSN 2576-6732
E-ISSN 2576-6724
Abstract
Christia vespertilionis (butterfly wing plant) is an ornamental plant originated from South East Asia with reported usage in traditional medicine practice
and potential as an anticancer and antitumor. This research aims to estimate the genome size of C. vespertilionis via flow cytometry (FCM) method. The
research was conducted with the optimisation of nuclear suspension preparation followed by the genome size estimation. Two chopping techniques
[manual chopping (MC) and BDTM Medimachine (MM)] and two lysis buffers (Otto and LBO1) were tested. Otto buffer with manual chopping was found to
be the most suitable method, generated fine DNA peak with minimum debris background, and coefficient of variation (CV) value less than 3%. Five replicates
of the FCM analysis were made for the genome size determination. The estimated genome size of C. vespertilionis was found to be 3.22 pg by using Glycine
max cv. Polanka (2C=2.5pg) as an external reference standard. Further comparison with other Christia species was not possible due to
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