Determination of the chromosome number and genome size of Garcinia mangostana L. via cytogenetics, flow cytometry and k-mer analyses

Midin, Mohd Razik and Nordin, Mohd Shukor and Madon, Maria and Saleh, Mohd Nazre and Hoe-Han, Goh and Mohd Noor, Normah (2018) Determination of the chromosome number and genome size of Garcinia mangostana L. via cytogenetics, flow cytometry and k-mer analyses. Caryologia, 71 (1). pp. 35-44. ISSN 0008-7114 E-ISSN 2165-5391

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Abstract

Mangosteen (Garcinia mangostana L.) is one of the most popular tropical fruit of South-East Asia. It has considerable economic potential for local and export markets. This paper describes a research work to determine the number of chromosomes and genome size of G. mangostana through chromosome counting, flow cytometry and k-mer analyses. Chromosome count analysis revealed that the chromosome number of G. mangostana varied from 74 to 110. The high number observed could be due to the occurrence of mutation and aneuploidy in G. mangostana. Using flow cytometry with Glycine max cv. Polanka (2C = 2.5 pg) used as standard, G. mangostana genome size was found to be 2C = 6.00 ± 0.17 pg. Meanwhile, a genome survey of G. mangostana was performed using Illumina HiSeq 2000 DNA sequencing; k-mer analysis revealed that the genome size of G. mangostana was approximately 5.92 Gbp, or approximately 6.05 pg (1 pg DNA = 0.9780 × 109 bp). Based on the flow cytometry and genome survey, the study concludes that the genome size of G. mangostana is between 6.00 and 6.05 pg.

Item Type:	Article (Journal)
Additional Information:	8764/89168
Uncontrolled Keywords:	Chromosome count; flow cytometry; genome size; G. mangostana; k-mer analysis
Subjects:	Q Science > QH Natural history > QH426 Genetics
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Science Kulliyyah of Science > Department of Biotechnology Kulliyyah of Science > Department of Plant Science
Depositing User:	Dr Mohd Razik Midin
Date Deposited:	07 Apr 2021 10:23
Last Modified:	07 Apr 2021 10:23
URI:	http://irep.iium.edu.my/id/eprint/89168

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