Development of HPLC method and quantification of amentoflavone from leaves extracts of three Calophyllum species

Aminudin, Nurul Iman and Norazhar, Nadia Aziba and Ahmad, Farediah (2021) Development of HPLC method and quantification of amentoflavone from leaves extracts of three Calophyllum species. In: IKM Pahang Branch Online Symposium 2021 : Chemistry for Sustainable World, 16 Jan 2021, Google Meet. (Unpublished)

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Abstract

This study described the development and validation of a method that can quantify amentoflavone in the methanol extracts of leaves of three Malaysian Calophyllum species via high performance liquid chromatography (HPLC) technique. Chromatographic analysis was conducted using a reverse phase C18 column with water and acetonitrile (55:45) as the mobile phase. The flow rate was 1.0 mL/min and detection by ultraviolet-visible detector at 270 nm. The developed method was validated in terms of linearity, precision and accuracy in accordance to International Conference on Harmonization (ICH) guidelines. The calibration curve showed good linearity (r2 > 0.9998) in a concentration range of 2.5 – 100 μg/mL with low limit of detection and limit of quantification at 1.33 and 4.02 μg/mL, respectively. The percentage of coefficient of variation for intra-day and inter-day precision was less than 2% while percentage of recovery was more than 90% indicating the precision and accuracy of the method. The developed HPLC method was proved as suitable and reliable for its intended application. The amount of amentoflavone quantified from C. incrassatum, C. canum and C. rubiginosum leaves extracts by using the developed method was 9.42, 30.39 and 24.23 μg/mL, respectively

Item Type:	Conference or Workshop Item (Slide Presentation)
Additional Information:	8125/89005
Uncontrolled Keywords:	amentoflavone, HPLC, quantification, Calophyllum, extracts
Subjects:	Q Science > QD Chemistry
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Science Kulliyyah of Science > Department of Chemistry
Depositing User:	Dr Nurul Iman Aminudin
Date Deposited:	23 Mar 2021 13:09
Last Modified:	23 Mar 2021 13:09
URI:	http://irep.iium.edu.my/id/eprint/89005

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