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Effect of pH on hematoxylin and eosin staining of rats intestinal tissue

Mohd Radzuan, Hazulin and Wan Omar, Wan Fatein Nabeila and Halim, Mohd Dhiyaulhaq (2020) Effect of pH on hematoxylin and eosin staining of rats intestinal tissue. In: Kuantan Research Day 2020: A National Online Event for E-poster Presentation, 1st October 2020 – 5th January 2021, Kuantan, Pahang. (Unpublished)

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Abstract

Background: Optimising hematoxylin and eosin (H&E) protocol does not only ensure reproducible and excellent staining quality, it also helps to optimise the use of lab materials and resources. This study investigates the effect of hematoxylin pH on H&E staining quality. Method: In an experimental study, 3 μm tissue sections of formalin-fixed paraffin embedded intestinal tissues of three healthy female Sprague-Dawley rats were subjected to the standard H&E protocol. Modified Gill’s hematoxylins of four pH (2.5, 2.75, 3.0 and 3.5) and eosin (pH 5.0) as counterstain were used. Results: At hematoxypin pH 2.5, the tissues appeared acidophilic with indistinctive epithelial lining. At pH 2.75, the hematoxylin dye was most prominent with good balance of staining, and crisp epithelial lining was observed. At pH 3.0 or higher, although the lining was crisp, blue-staining mucin were observed. Conclusion: pH 2.74 is the most optimal pH to achieve balance of coloration and definitive epithelial lining in H&E staining.

Item Type: Conference or Workshop Item (Poster)
Additional Information: 6245/88588 Presented via Online
Uncontrolled Keywords: Histology technique, hematoxylin and eosin, pH, epithelial lining, mucin
Subjects: Q Science > Q Science (General)
Q Science > QD Chemistry
R Medicine > R Medicine (General)
R Medicine > RB Pathology
R Medicine > RZ Other systems of medicine
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Medicine
Kulliyyah of Medicine > Department of Basic Medical
Depositing User: Dr Hazulin Mohd Radzuan
Date Deposited: 27 Feb 2021 22:04
Last Modified: 27 Feb 2021 22:04
URI: http://irep.iium.edu.my/id/eprint/88588

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