Characterization of a 2,2-dichloropropionic acid (2,2-dcp) degrading alkalotorelant Bacillus megaterium strain BHS1 isolated from Blue Lake in Turkey

Abdul Wahhab, Batool Hazim and Khairul Anuar, Nurul Fatin Syamimi and Abdul Wahab, Roswanira and Samsulrizal, Nurul Hidayah and Al-Nimer, Marwan S.M and Abdul Hamid, Azzmer Azzar and Edbeib, Mohamed Faraj and Kaya, Yilmaz and Huyop, Fahrul Zaman (2021) Characterization of a 2,2-dichloropropionic acid (2,2-dcp) degrading alkalotorelant Bacillus megaterium strain BHS1 isolated from Blue Lake in Turkey. In: International Conference By Biotechnology Society Of Nepal (ICBSN-2021), 12th-14th February 2021, Bhaktapur, Nepal. (Unpublished)

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Abstract

An acid, 2,2-dichloropropionic acid (2,2-DCP) is an active ingredient in herbicide (Dalapon®). Using 2,2-DCP as a model substrate, an alkalotolerant bacterium was successfully isolated from the Blue Lake, Turkey. This bacterium is a potential bioremediation agent of recalcitrant xenobiotic halogenated compounds. This study aimed to prove the efficacy of the alkalotolerance Bacillus megaterium BHS1 in degrading 2,2-DCP as the sole source of carbon. Biolog GEN III system and 16S rRNA analysis were used for the identification of the bacterium. It was discovered that the strain BHS1 is Bacillus megaterium, and the bacterium that was observed to thrive in alkaline conditions (pH 7.0−14.0), supplemented with varying concentrations of 2,2-DCP (from 20 to 60 mM). Growth of strain BHS1 was exceptional in 40 mM of 2,2-DCP at pH 9, corresponding to a cell doubling time of 17.7 hour, whereas was fully inhibited at 50 mM 2,2-DCP. Since halogenated pollutants can make their way into highly alkaline environments, therefore, identifying threshold levels of strain BHS1 with respect to alkaline-tolerance and maximum level of 2,2-DCP may prove pertinent. This is to ensure that an optimal environment is created for the bacteria to degrade 2,2-DCP-contaminated water. In addition, this is the first study exploring a Bacillus species isolated from an alkaline environment adept in utilizing 2,2-DCP as a sole source of carbon. Hence, the ability of this strain to degrade other types of haloalkanoic acids constitutes a worthy future study.

Item Type:	Conference or Workshop Item (Speech/Talk)
Additional Information:	8022/ Presented via online
Subjects:	Q Science > QR Microbiology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Science > Department of Plant Science Kulliyyah of Science Kulliyyah of Science > Department of Biotechnology
Depositing User:	Dr Nurul Hidayah Samsulrizal
Date Deposited:	02 Mar 2021 13:44
Last Modified:	02 Mar 2021 13:44
URI:	http://irep.iium.edu.my/id/eprint/88381

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