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Seroconversion of indirect immunofluorescent antibody IgM and IgG in melioidosis

Arumugam, Janaki and How, Soon Hin and Zainulabid, Ummu Afeera and Mustafa Mahmoud, Mohammed Imad Al-Deen and Ab Rahman, Jamalludin and Ab Rahman, Ahmad Kashfi and Kuan, Yeh Chunn (2020) Seroconversion of indirect immunofluorescent antibody IgM and IgG in melioidosis. Bangladesh Journal of Infectious Diseases, 7 (2). pp. 61-66. ISSN 2411-4820 E-ISSN 2411-670X

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Background: There are several serological tests used in the diagnosis of melioidosis. However, the interpretation of the results can be problematic in endemic areas because there might be a high background positivity due to previous exposure. Objective: This study aimed to determine the usefulness of Indirect Immunofluorescent Antibody (IFA) IgM and IgG in the diagnosis of melioidosis and the time for seroconversion. Methodology: We prospectively studied the trend of IFA IgM and IgG in 40 patients with culture-confirmed melioidosis over three months at six different time points (days 1, 8, 15, 30, 60, and 90). Results: From the results, 37.5% and 32.5% of patients had IFA IgM and IgG of ≤ 1:20 respectively on day 1 when the blood culture was positive. The natural log (ln) of the titres was used for the analysis. Repeated measures ANOVA showed significant changes in both IgM (p= 0.001) and IgG (p= 0.045) respectively throughout six sampling time points. Both the means of ln IgM and ln IgG peaked at day 15. At day 90, mean ln IgG remained high but mean ln IgM dropped to a level below that of day one. All culture-confirmed melioidosis patients recorded a maximum IFA IgM titre of at least 1: 80, but all subsequently dropped to below this level at day 90. Conclusion: IFA IgM is a more useful diagnostic serological marker than IFA IgG in acute melioidosis.

Item Type: Article (Journal)
Additional Information: 7459/88026
Uncontrolled Keywords: melioidosis, serology, IFA IgM, IFA IgG, seroconversion
Subjects: R Medicine > RC Internal medicine
R Medicine > RC Internal medicine > RC111 Infectious and Parasitic Diseases
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Medicine
Kulliyyah of Medicine > Department of Basic Medical
Kulliyyah of Medicine > Department of Community Medicine (Effective: 1st January 2011)
Kulliyyah of Medicine > Department of Internal Medicine
Depositing User: Asst. Dr. Ummu Afeera Zainulabid
Date Deposited: 25 Jan 2021 16:49
Last Modified: 25 Jan 2021 16:49
URI: http://irep.iium.edu.my/id/eprint/88026

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