LPS-induced apoptosis is partially mediated by hydrogen sulphide in RAW 264.7 murine macrophages

George, Leema and Ramasamy, Tamizhselvi and Kuttulebbai Naina Mohamed Salam, Sirajudeen and Manickam, Venkatraman (2019) LPS-induced apoptosis is partially mediated by hydrogen sulphide in RAW 264.7 murine macrophages. Immunological Investigations, 48 (5). pp. 451-465. ISSN 0882-0139 E-ISSN 1532-4311

	PDF (Article) - Published Version Restricted to Repository staff only Download (2MB) | Request a copy
	PDF (SCOPUS) - Published Version Restricted to Repository staff only Download (491kB) | Request a copy

Abstract

Abstract Lipopolysaccharide (LPS) induces apoptosis in murine macrophages through the autocrine secretion of tumor necrosis factor (TNF)-α and nitric oxide (NO). LPS-induced inflammation in murine macrophages is associated with hydrogen sulfide (H2S) production. In this present study, we reported the novel role of H2S in LPS-induced apoptosis and its underlying molecular mechanism specifically at late phases in murine macrophage cells. Stimulation of RAW 264.7 macrophages with LPS resulted in a time- and dose-dependent induction of apoptosis. We observed that the LPS-induced early apoptosis (associated with TNF-α secretion) in macrophages was not inhibited in the presence of H2S inhibitor (DL-propargylglycine), whereas early apoptosis was absent in the presence of TNF receptor antibody. Interestingly, LPS-induced late apoptosis paralleled with H2S production was reduced in the presence of H2S inhibitor but not with TNF receptor antibody. The late apoptotic events mediated by H2S and not the TNF-α induced early apoptosis correlated significantly with the induction of p53 and Bax expression in LPS-induced macrophages. Thus, it is possible that RAW 264.7 murine macrophages treated with LPS mediated early apoptosis through TNF-α and the late apoptotic events through the production of H2S.

Item Type:	Article (Journal)
Additional Information:	9547/87629
Uncontrolled Keywords:	LPS, TNFα, H2S, cystathionine γ-lyase, apoptosis
Subjects:	R Medicine > R Medicine (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Medicine Kulliyyah of Medicine > Department of Basic Medical
Depositing User:	Prof.Dr. SIRAJUDEEN KUTTULEBBAI NAINAMOHAMED SALAM
Date Deposited:	11 Jan 2021 09:41
Last Modified:	11 Jan 2021 09:41
URI:	http://irep.iium.edu.my/id/eprint/87629

Actions (login required)

View Item