Steroidogenic effects of Eurycoma longifolia standardized extract in rat model of polycystic ovarian syndrome

Abdul Rahman, Suzanah and Ahmad, Nur Amalina and Mamat, Suhana and Chan, Kit Lam (2020) Steroidogenic effects of Eurycoma longifolia standardized extract in rat model of polycystic ovarian syndrome. Journal of Pharmacy and Bioallied Sciences, 12 (Suppl. 2). S906-S907. ISSN 0976-4879 E-ISSN 0975-7406

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Abstract

Introduction: Female reproductive disorders are multifaceted in nature and the treatment of polycystic ovarian syndrome has remained a challenge in the gynaecological field. Objectives: Eurycoma longifolia has long been used for enhancement of male reproductive functions. This study sought to elucidate its effects in the female reproductive system. Materials and Methods: Eurycoma longifolia standardized extract, TAF 273, was tested on rats representing the polycystic ovarian syndrome (PCOS) following induction with a single subcutaneous injection of estradiol valerate (2 mg/rat). At the end of two moths, clomiphene citrate and TAF 273 (25, 50 and 100 mg/kg) were administered daily via oral gavage for 28 consecutive days. Serum testosterone, oestradiol, progesterone, luteinizing hormone and follicle stimulating hormone levels were then measured followed by assessment of ovary histomorphology. Steroidogenic enzymes were also examined in histological paraffin sections using HRP-DAB detection method. Results: The expression levels of CYP11A1, CYP17A1 and CYP19A1 genes were detected by quantitative RT-PCR. TAF 273 extract at 100 mg/kg was found to possess beneficial effects in improving the PCOS condition by restoration of the oestrous cycle and improvement of the ovarian morphology as observed by the presence of healthy follicles and corpora lutea indication the process of ovulation. Hormonal imbalances and expressions of steroidogenic genes were also found to be attenuated probably through its action on oestradiol hormones and CYP19A1 gene. Conclusion: Results obtained showed that the standardized extract has the potential in maintaining normal functions of the female reproductive system and may have economic value in the management of female disorders and reproductive health.

Item Type:	Article (other)
Uncontrolled Keywords:	Eurycoma longifolia, estradiol valerate, female reproductive disorder, polycystic ovarian syndrome, steroidogenic enzymes
Subjects:	Q Science > QP Physiology R Medicine > RG Gynecology and obstetrics R Medicine > RG Gynecology and obstetrics > RG125 Therapeutics R Medicine > RG Gynecology and obstetrics > RG133.5 Reproductive technology > RG133.7 Ovulation induction R Medicine > RM Therapeutics. Pharmacology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Allied Health Sciences Kulliyyah of Allied Health Sciences > Department of Biomedical Science (Effective:1st July 2011)
Depositing User:	Prof. Dr. Suzanah Abdul Rahman
Date Deposited:	27 Dec 2023 12:24
Last Modified:	27 Dec 2023 16:14
URI:	http://irep.iium.edu.my/id/eprint/87060

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