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Reduced graphene oxide on screen-printed carbon electrodes as biosensor for escherichia coli O157:H7 detection

Barthasarathy, Piravin Raj and Ahmed, Nasteho Ali and Wan Salim, Wan Wardatul Amani (2020) Reduced graphene oxide on screen-printed carbon electrodes as biosensor for escherichia coli O157:H7 detection. MDPI, 60 (1). pp. 1-8. E-ISSN 1996-1073

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Mixture of drinking-water supplies with sewage discharges poses disease threats in flood-stricken areas. In such exigent conditions, on-site testing of water samples is the only option, as water samples cannot be transported to laboratories owing to severely impacted transportation services. Hence, we developed a low-cost electrochemical biosensor fabricated from a screen-printed carbon electrode (SPCE) to detect E. coli O157:H7, a virulent pathogen often found in sewage discharges. We focused on understanding antigen-antibody interaction when the antibody used is not specific for E. coli O157:H7. We found that antibody immobilized on a reduced graphene oxide (rGO)–modified SPCEs distinguished between E. coli O157:H7 concentrations of 4 × 108 and 4 CFU/ml, with lowest current reported for 4 × 108 CFU/ml. In contrast, a reduced graphene oxide–modified SPCEs without antibody immobilization does not produce a prominent peak that distinguishes the highest and lowest E. coli concentrations. However, a few E. coli cells were still attached to the rGO/SPCEs in the absence of antibody, as shown in FESEM images. A processing step of differential readings from reference and active electrodes needs to be programmed into an Arduino® microprocessor to realize a prototype of a bacteria sensor for field use.

Item Type: Article (Journal)
Additional Information: 7116/85179
Uncontrolled Keywords: screen-printed carbon electrode; E. coli O157:H7; reduced graphene oxide; electrochemical biosensor; water quality monitoring
Subjects: T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Engineering
Kulliyyah of Engineering > Department of Biotechnology Engineering
Depositing User: Dr Wan Wardatul Amani Wan Salim
Date Deposited: 01 Dec 2020 17:13
Last Modified: 01 Dec 2020 17:13
URI: http://irep.iium.edu.my/id/eprint/85179

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