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The role of polyamines in beta vulguris (beetroot), zizhipus jujube (jujube) and vitis venefera (raisin) on human lung adenocarcinoma cells (A549) proliferation and apoptosis

Jamil, Elyna Fatini and Abd Nadzir, Nur Sarah Nabila and Abdul Ghani, Radiah (2020) The role of polyamines in beta vulguris (beetroot), zizhipus jujube (jujube) and vitis venefera (raisin) on human lung adenocarcinoma cells (A549) proliferation and apoptosis. Journal of Pharmacy and Bioallied Science, 12 (6 (Supplement)). S928-S928. ISSN 0975-7406

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Abstract

Introduction: Polyamines are vital in maintaining human health because they are crucial for cell growth. Excessive intracellular polyamines in diet may promote unwarranted cell proliferation. Objective: This study aimed to investigate polyamines as a chemopreventive agent in selected fruits against human lung adenocarcinoma cells, A549. Materials and Methods: Beta vulgaris (beetroot), Ziziphus jujube (jujube) and Vitis vinifera (raisin) were freeze dried and kept at -80 °C until analysis. The anti-proliferative activity of selected fruits was assessed on A549 cells using MTT assay and the effect on A549 growth was completed via Trypan bluedye exclusion assay. Cell cycle profile and apoptosis were conducted with flow cytometer, while caspase assay was done using microtiter plate-reader. The quantification of intracellular polyamines was done using HPLC and the effect of these medicinal plants on the gene expression of selected polyamine metabolic enzymes was determined using qPCR. Results: The IC50 ranged between 15 to 30 mg/ml for these three fruits. A549 growth indicated significant decreased in cell viability after 48 h. Annexin-V/PI demonstrated significant increase in early apoptosis in beetroot treated cells while other fruits caused significant increase in late apoptosis at 48 h of treatments. There was a significant downregulation of ODC gene in cells treated with jujube and raisins while upregulation of SSAT gene in beetroot treated cells. Conclusion: These medicinal fruits are the promising candidates for food-based chemoprevention strategy for their low polyamine content, vital in modulating polyamine metabolism and promoting cell death.

Item Type: Article (Meeting Abstract)
Additional Information: 4458/84765
Uncontrolled Keywords: Lung cancer; nutritional cancer therapy; prophetic medicinal fruits; polyamines
Subjects: R Medicine > RM Therapeutics. Pharmacology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Allied Health Sciences > Department of Biomedical Science (Effective:1st July 2011)
Kulliyyah of Allied Health Sciences
Depositing User: Dr Radiah Abdul Ghani
Date Deposited: 23 Nov 2020 15:16
Last Modified: 23 Nov 2020 15:16
URI: http://irep.iium.edu.my/id/eprint/84765

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