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Inhibition of Streptococcus pneumoniae hyaluronidase by honeys of Malaysian origins

Mohd Nayian, Nurul Azahana and Ahmad Yusof @ Hanafi, Hanani (2020) Inhibition of Streptococcus pneumoniae hyaluronidase by honeys of Malaysian origins. Bioremediation Science and Technology Research, 8 (1). pp. 1-6. E-ISSN 2289-5892

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Streptococcus pneumoniae, a major human pathogen causing meningitis, pneumonia and bacteraemia produces hyaluronidase as its virulence factor. This enzyme increases the bacterial permeability to the host tissue by degrading hyaluronic acids that maintain the viscosity of connective tissues. Limited hyaluronidase inhibitors are available at the moment, thus leading us to identify potential hyaluronidase inhibitors from local products such as honeys. Three local honeys (Tualang, Kelulut and Gelam) have been selected and undergo hyaluronidase inhibition test through hyaluronic acids turbidity reduction assay. Honey that shows the highest and lowest anti-hyaluronidase was analysed for flavonoid and phenolic content, to identify association between the contents and hyaluronidase inhibition rate. All honeys showed various degrees of inhibition against hyaluronidase of S. pneumoniae where honey with the highest antihyaluronidase activity is Kelulut with 18% inhibition, while honey with the lowest antihyaluronidase activity is Gelam with 2% inhibition. Kelulut also demonstrated highest phenolic content, where significant association was found between the inhibition and the content. Therefore, this study supported the claim that higher phenolic contents indicate greater inhibitory activity on hyaluronidase enzyme. The findings suggest that local Malaysian honey have potential as hyaluronidase inhibitor which could be beneficial for future treatment against S. pneumoniae infections.

Item Type: Article (Journal)
Additional Information: 7232/84411
Uncontrolled Keywords: Streptococcus pneumoniae, hyaluronidase inhibition, kelulut, flavonoid
Subjects: Q Science > QR Microbiology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Allied Health Sciences
Kulliyyah of Allied Health Sciences > Department of Biomedical Science (Effective:1st July 2011)
Depositing User: Dr Hanani Ahmad Yusof @ Hanafi
Date Deposited: 11 Nov 2020 10:28
Last Modified: 11 Nov 2020 10:28
URI: http://irep.iium.edu.my/id/eprint/84411

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