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Reduced graphene oxide on screen-printed carbon electrodes as biosensor for Escherichia coli O157:H7 detection

Barthasarathy, Piravin Raj and Wan Salim, Wan Wardatul Amani (2020) Reduced graphene oxide on screen-printed carbon electrodes as biosensor for Escherichia coli O157:H7 detection. In: The 1st International Electronic Conference on Biosensors (IECB 2020), 02nd-17th November 2020, Online platform.

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Rapid development and deployment of biosensors for pathogen quantification is needed for flood-stricken areas. Drinking-water supplies in flooded areas become a disease threat owing to mixture with sewage discharge. In such exigent conditions, water is filtered through a microfiltration technique that is moderately effective in removing bacteria and non-effective in removing viruses. Quantification of pathogens using biochemical techniques does not provide real-time data and requires transportation of water samples to laboratories for analysis. However, transportation services are severely affected during flooding, and hence on-site testing may be the only option. WHO specifies that diagnostic devices are to be made ASSURED – affordable, sensitive, specific, user-friendly, rapid and robust, equipment-free, and deliverable to end-users. To fulfil ASSURED criteria, we developed a low-cost electrochemical biosensor integrated with an Arduino® microprocessor encased in a portable platform for E. coli O157:H7 quantification. The working electrode (WE) of a screen-printed carbon electrode (SPCE) was modified with reduced graphene oxide (rGO). Rabbit serum IgG, a non-specific antibody towards E. coli O157:H7, was immobilized on the WE. We tested the specificity of E. coli O157:H7 binding to IgG/rGO/SPCEs and rGO/SPCEs at 4 to 4 × 108 CFU/ml. We chose a potential range of -0.35 to 0.07 in LSV to compare the current values at different concentrations. At -0.14V, IgG/rGO/SPCEs distinguished E. coli concentrations of 4 and 4 × 108 CFU/ml with current value of 17 µA and 1 µA, respectively. However, the IgG/rGO/SPCE does not distinguish between E. coli concentration of 4 × 107 and 4 × 108 CFU/ml. The rGO/SPCE does not distinguish E. coli concentration of 4 and 4 × 108 CFU/ml, as evidenced by almost similar current values in both concentrations. This study indicates the potential of non-specific antibodies for E. coli O157:H7 quantification during water-quality monitoring in flooded areas.

Item Type: Conference or Workshop Item (Plenary Papers)
Additional Information: 7116/84241
Uncontrolled Keywords: screen-printed carbon electrode, E. coli O157:H7, reduced graphene oxide, electrochemical biosensor, water quality monitoring
Subjects: Q Science > QR Microbiology
T Technology > TP Chemical technology > TP248.13 Biotechnology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Engineering > Department of Biotechnology Engineering
Kulliyyah of Engineering
Depositing User: Dr Wan Wardatul Amani Wan Salim
Date Deposited: 04 Nov 2020 10:57
Last Modified: 04 Nov 2020 10:57
URI: http://irep.iium.edu.my/id/eprint/84241

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