Awang, Nursyahidatul Azwa and Amid, Azura and Arshad, Zatul Iffah
(2020)
Method for purification of collagen: A systematic review.
Asia Pacific Journal of Molecular Biology & Biotechnology, 28 (3).
pp. 99-112.
ISSN 0128-7451
E-ISSN 2672-7277
Abstract
Collagen has diverse applications in various industries and thus a various method of
purifications has been developed over the years to produce high yield and high purity of collagen to fulfill
collagen market demand. The objective of this qualitative systematic review is to summarize the current
literature on the existing purification methods for collagen from different sources. Toward this end, three
databases were searched and articles were screened for all original articles explaining the purification of
collagen regardless of their main sources. After the comprehensive screening, out of 715 articles, 12 articles
were chosen and were further reviewed. The analysis of the technical details, theory, advantages, and
disadvantages of the techniques used are reported in this study. We found four types of purification
methods that commonly used to purify various types of collagen from mammals, marine, bacteria, and
recombinant collagen. The correlations between the prevalence of the method and the efficiency of collagen purification were also identified. It highlighted that many factors should be considered before choosing any method of purification such as types, sources and structure of the collagen itself. Affinity
chromatography commonly used for purification of recombinant collagen as they have been genetically modified and appended by affinity tags whereas conventional purification method is preferable for nonrecombinant collagen from mammalian and marine sources.
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