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Developmental toxicity evaluation of sub-CO2 extract of Phaleria macrocarpa fruit flesh in Danio rerio embryo

Mia, Md. Abdur Rashid and Sarker, Md. Zaidul Islam and Ferdosh, Sahena and Ahmed, Qamar Uddin and Uddin, A.B.M Helal and Abul Kalam, Azad (2020) Developmental toxicity evaluation of sub-CO2 extract of Phaleria macrocarpa fruit flesh in Danio rerio embryo. In: International Research Conference on Pharmaceutical and Allied Sciences, 7-8th March March 2020, Kuala Lumpur, Malaysia. (Unpublished)

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Abstract

Phaleria macrocarpa, traditionally known as Mahkota dewa, is a therapeutic herb that is being used for anti-obesity, antidiabetic, antioxidant, and anticancer activities. To perform animal study and administration dose measurement, to identify the safety profile level of plant extract is very important. The main aim of this study is to evaluate the toxicity level of the subcritical carbon dioxide (Sub-CO2) extract in Danio-rerio embryo. Fruit was chosen and subsequently extracted using Sub-CO2 technique. The Box-Behnken Design (BBD) was used to optimize and examine the effect of independent variables of Sub-CO2 such as temperature (27-29˚C), pressure (6.8-7.0 MPa) and concentration of co-solvent. The toxicity study of Sub-CO2 extracts was examined by using Danio-rerio embryo at 0 to 96 hpf. The protocol of toxicity study on zebrafish embryo was adapted according to OECD guideline section 212.Sub-CO2 extract of P. macrocarpa was applied to screen the toxicity level at the dose of 100 – 1000 µg/ml. The tested report showed considerable (>90%) mortality and delay in hatching at higher concentrations of 1000 µg/ml. Overall, there was a mild toxicity observed in experimental embryo development when compared to control. However, the LC50 value was obtained at the concentration of 841.39 µg/ml. Toxicity study of different ratio of Sub-CO2 extracts from P. macrocarpaa showed mild toxic effects on zebrafish embryos, however, the extract comparatively safe for normal embryonic development at the range from 50 to 841.39 µg/ml. This finding could help to predetermine the dosage P. macrocarpa fruit for further in vivo study

Item Type: Conference or Workshop Item (Poster)
Additional Information: 7165/82944
Uncontrolled Keywords: Subcritical CO2 extract; Zebrafish embryo; Phaleria macrocarpa fruit; co-solvent; toxicity
Subjects: Q Science > Q Science (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Pharmacy
Kulliyyah of Science
Kulliyyah of Science > Department of Plant Science
Depositing User: Dr. Sahena Ferdosh
Date Deposited: 28 Sep 2020 15:15
Last Modified: 10 Nov 2020 16:22
URI: http://irep.iium.edu.my/id/eprint/82944

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