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Phytochemical and cytotoxicity evaluation of lagerstroemia speciosa (L.) leaves extract by MCF-7 cell line and brine shrimp lethality bioassay

Azad, Abul Kalam and Helaluddin, Abul Bashar Mohammed (2020) Phytochemical and cytotoxicity evaluation of lagerstroemia speciosa (L.) leaves extract by MCF-7 cell line and brine shrimp lethality bioassay. International Journal of Drug Delivery Technology, 10 (1). pp. 40-44. ISSN 0975-4415

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Abstract

This study aimed to investigate the cytotoxicity of Lagerstroemia speciosa (L.) leaves crude extract. It has been reported to show various bioactivities. The phytochemical screening of the extract has been carried qualitatively. The cytotoxic effect was determined through in vitro MTT assay of MCF-7 cell line, and brine shrimp lethality bioassay. The presence or absence of alkaloid, carbohydrate, glycoside, saponin, terpene, steroid, phenol, and flavonoid in the extract was determined through the qualitative tests. The extract showed cell viability of 100% (1.95–3.9 μL/mL), 96% (1.95 μL/mL), ≈ 95% (3.9–15.62 μL/ mL) and 88% (250 μL/mL) while the mortality of brine shrimp nauplii was from 5% to 10% (7.8 – 125 μL/mL) respectively. For both assays, DMSO of 1 & 0.1% were used as vehicle controls, while the potassium dichromate as the positive control for the brine shrimp only. These results proved the leaves extract to be non-toxic.

Item Type: Article (Journal)
Additional Information: 4478/82575
Uncontrolled Keywords: Brine shrimp nauplii, Crude extract
Subjects: Q Science > QD Chemistry
R Medicine > RM Therapeutics. Pharmacology
R Medicine > RM Therapeutics. Pharmacology > RM300 Drugs and their action
R Medicine > RS Pharmacy and materia medica
R Medicine > RS Pharmacy and materia medica > RS192 Materia Medica-Pharmaceutical Technology
R Medicine > RS Pharmacy and materia medica > RS403 Materia Medica-Pharmaceutical Chemistry
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Pharmacy
Depositing User: Dr. A. B. M. Helal Uddin
Date Deposited: 10 Sep 2020 16:12
Last Modified: 10 Sep 2020 16:12
URI: http://irep.iium.edu.my/id/eprint/82575

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