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In vitro evaluation of poly (lactic acid-co-glycolic acid)/ atelocollagen/fibrin bioscaffold for annulus fibrosus regeneration

Mohamad, Mohd Yusof and Mohamed Amin, Muhammad Azri Ifwat and Zulkifly, Ahmad Hafiz and Sha'ban, Munirah (2020) In vitro evaluation of poly (lactic acid-co-glycolic acid)/ atelocollagen/fibrin bioscaffold for annulus fibrosus regeneration. Trends in Biomaterials and Artificial Organs, 34 (3). pp. 118-123. ISSN 09711198

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Abstract

The current study evaluated the 3D culture of the PLGA incorporated with atelocollagen and fibrin seeded with the annulus cells of intervertebral disc. The evaluation was performed in three consecutive weeks observing at the morphology, compression modulus, histology, immunohistology, sulphated glycosaminoglycan (SGAG) and DNA content. A comparison was made between the AF seeded onto the PLGA (PLGA-AF), PLGA with atelocollagen (PAAF), PLGA with fibrin (PF-AF) and PLGA with both atelocollagen and fibrin (PAF-AF). The constructs displayed glistening morphology with high wet weight and cellular viability by week 3. The PAF-AF and PA-AF demonstrated significant compression modulus. The presence of collagen type I were detected in all the constructs. The histological findings showed extensive AF cellular distribution. Significant quantitative sGAG was detected for the PAF-AF constructs but with no significant differences on the DNA content. Overall, the PAF-AF constructs support the cellular proliferation of the AF

Item Type: Article (Journal)
Additional Information: 8264/82477
Subjects: R Medicine > R Medicine (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Allied Health Sciences
Kulliyyah of Allied Health Sciences > Department of Biomedical Science (Effective:1st July 2011)
Kulliyyah of Allied Health Sciences > Department of Physical Rehabilitation Sciences
Kulliyyah of Medicine
Kulliyyah of Medicine > Department of Department of Orthopaedics, Traumatology & Rehabilitation
Depositing User: Dr Mohd Yusof Mohamad
Date Deposited: 01 Sep 2020 09:37
Last Modified: 01 Sep 2020 09:39
URI: http://irep.iium.edu.my/id/eprint/82477

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