Trihoney reduces lipid peroxidation and enhances antioxidant enzyme activities in hypercholesterolaemic atherosclerotic rabbits

Hamad Alfarisi, Hamad Abdulsalam and Ibrahim, Muhammad and Hamad Mohamed, Zenab and Hamdan, Asmah Hanim and Che Mohamad, Che Anuar (2020) Trihoney reduces lipid peroxidation and enhances antioxidant enzyme activities in hypercholesterolaemic atherosclerotic rabbits. International Food Research Journal, 27 (3). pp. 568-575. E-ISSN 2231-7546

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Abstract

Oxidative stress and reactive oxygen species (ROS) constitute a major pathogenic mechanism for the development of atherosclerosis. In the present work, the antioxidant potential of Trihoney was investigated in hypercholesterolaemic rabbits. Thirty-six male New Zealand white (NZW) rabbits were grouped into: normal diet (C), normal diet with 0.6 g/kg/day of Trihoney (C+H), 1% cholesterol diet (HCD), 1% cholesterol diet with 0.3 g/kg/day of Trihoney (HCD+H1), 1% cholesterol diet with 0.6 g/kg/day of Trihoney (HCD+H2), and 1% cholesterol diet with 2 mg/kg/day of atorvastatin (HCD+At.). Animals were sacrificed following 12 weeks of treatment, and their serum was analysed for oxidised-low density lipoprotein (Ox-LDL). Serum and aortic tissue homogenate were assayed for superoxide dismutase (SOD), glutathione peroxidase (GPx), and malondialdehyde (MDA). Hypercholesterolemia caused a significant (p < 0.05) elevation in serum Ox-LDL and a significant (p < 0.05) reduction of antioxidant enzyme activities in serum of the HCD group. Trihoney induced a significant (p < 0.05) increase in antioxidant enzyme activities in serum as compared to the HCD group. The high cholesterol diet suppressed both antioxidant enzymes in aortic homogenate. Trihoney significantly (p < 0.05) enhanced both antioxidant enzymes in aortic homogenate. Hypercholesterolemia induced a significant (p < 0.05) elevation of serum lipid peroxidation in the HCD group. Trihoney caused a significant (p < 0.05) reduction of lipid peroxidation in aortic homogenate. These results demonstrated that Trihoney has the potential to ameliorate oxidative stress systemically, as well as locally in the atherosclerotic aorta.

Item Type:	Article (Journal)
Additional Information:	4669/82073
Uncontrolled Keywords:	atorvastatin, hypercholesterolemia, oxidative stress, Trihoney
Subjects:	Q Science > Q Science (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Allied Health Sciences
Depositing User:	DR ASMAH HANIM HAMDAN
Date Deposited:	17 Aug 2020 09:07
Last Modified:	21 Nov 2023 09:24
URI:	http://irep.iium.edu.my/id/eprint/82073

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