Preliminary study on the effect of Tualang honey and its silver nanoparticles on Kainic acid mediated behavioural changes in male rats

Hasim, Hidani and Zulkifli, Nurdarina Ausi and Asari, Mohd Asnizam and Muthuraju, Sangu and Visweswara Rao, Pasupuleti Visweswara and Mustapha, Muzaimi and Kuttulebbai Naina Mohamed Salam, Sirajudeen (2020) Preliminary study on the effect of Tualang honey and its silver nanoparticles on Kainic acid mediated behavioural changes in male rats. Malaysian Journal of Medical Sciences, 27 (Special Issue). pp. 18-19. ISSN 1394-195X E-ISSN 2180-4303

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Abstract

Objectives: Excitotoxicity is a key mechanism in neurodegenerative diseases. Kainic acid (KA) is used to induce experimental excitotoxicity. Therefore, the present study investigates the effect of Tualang honey (TH) and its silver nanoparticles (THSN) on KA mediated behavioural changes in rats. Methods: Adult Sprague-Dawley male rats (n = 18) were randomised into six groups (n = 3/group): i) Control, ii) THSN, iii) KA, iv) TH + KA, v) THSN + KA and vi) Topiramate + KA groups which were pre-treated orally with distilled water, THSN (50 mg/kg body weight), Tualang honey (1.0 g/kg body weight) or Topiramate (40 mg/kg body weight), respectively, five times at 12 h intervals. Saline or KA (15 mg/kg body weight) were injected subcutaneously 30 min after last oral treatment. The latency to onset of the first generalised seizures (FGS) was recorded. Behavioural assessments such as open field test (OFT) to assess the locomotor activity and novel object recognition test (NORT) for learning and memory were performed after 24 h of KA administration. Results: On KA treatment, FGS was observed. But there is a trend of amelioration of FGS on TH+KA and THSN+KA groups when compared to KA group. Pretreatment with TH and THSN improved learning and memory as evidenced by increased recognition index in NORT when compared to untreated KA group. Interestingly, OFT results of THSN+KA group improved the locomotor activity when compared to untreated KA group. Conclusion: These findings suggest that pre-treatment with TH and THSN provides protective effect on KA mediated behavioural changes in male rats. However, further study required to understand the mechanism of action.

Item Type:	Article (Meeting Abstract)
Additional Information:	9547/81960
Uncontrolled Keywords:	kainic acid, honey, metal nanoparticles, Sprague-Dawley rats, learning
Subjects:	R Medicine > R Medicine (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Medicine Kulliyyah of Medicine > Department of Basic Medical
Depositing User:	Prof.Dr. SIRAJUDEEN KUTTULEBBAI NAINAMOHAMED SALAM
Date Deposited:	04 Aug 2020 11:15
Last Modified:	11 Jan 2021 12:47
URI:	http://irep.iium.edu.my/id/eprint/81960

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