New lipase producing Betaproteobacteria strains of Caldimonas sp. and Tepidimonas sp. isolated from a Malaysian hotsprings

Mohd Ghazali, Sharul Azim and Tengku Abdul Hamid, Tengku Haziyamin (2016) New lipase producing Betaproteobacteria strains of Caldimonas sp. and Tepidimonas sp. isolated from a Malaysian hotsprings. Malayan Nature Journal, 68 (Part 1 & 2). pp. 83-90. ISSN 00251292

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Abstract

Microbial lipolytic enzymes have attracted considerable attention owing to their biotechnological potential. In this study, slightly thermophilic bacteria producing lipase were isolated from Bentong and Sungai Lembing hot springs, Pahang, Malaysia. Out of 25 colonies isolated, 14 samples showed to produce clear zones surrounding the growth on tributyrin agar plates. All 14 isolates showed Gram-negative bacteria with short rod morphology. PCR amplification of 16S ribosomal DNA gene showed that these isolates were clustered with subclass Betaproteobacteria consisting of slightly thermophilic bacteria that produce lipase. Phylogenetic analysis was carried out with the highly similar species and four isolates (SglA1, BtnC1, BtnC2 and BtnC3) are related to genus Caldimonas and 10 isolates (SglB1, SglB2, SglB3, SglB4, BtnB1, BtnB2 BtnD1, BtnD2, BtnD3 and BtnD4) belonged to Tepidimonas. These results indicated that novel lipase-producing thermophilic Betaproteobacteria could be isolated from these hot springs.

Item Type:	Article (Journal)
Additional Information:	4261/81879
Uncontrolled Keywords:	slightly thermophilic, betaproteobacteria, thermostable lipase, hot spring
Subjects:	Q Science > QR Microbiology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Science > Department of Biotechnology
Depositing User:	Dr Tengku Haziyamin Tengku Abd Hamid
Date Deposited:	04 Aug 2020 11:18
Last Modified:	04 Aug 2020 11:18
URI:	http://irep.iium.edu.my/id/eprint/81879

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