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Modulation of metabolic alterations of obese diabetic rats upon treatment with Salacca zalacca fruits extract using H NMR-based metabolomics

Saleh, Mohammed S M and Siddiqui, Mohammad Jamshed Ahmad and Mediani, Ahmed and Ahmed, Qamar Uddin and Mat So'ad, Siti Zaiton and Saidi-Besbes, Salima and Elnaem, Mohamed Hassan and Othman, Hairol Azrin and Ismail, Nor Hadiani (2020) Modulation of metabolic alterations of obese diabetic rats upon treatment with Salacca zalacca fruits extract using H NMR-based metabolomics. Food Research International, 137. ISSN 0963-9969

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Abstract

Fruit of salak (Salacca zalacca) is tradition ally used and commercialized as an an tidiabetic agent. How ever, the scientific evidence to prove this traditional use is lack ing. This re search was aimed to evaluate the metabolic changes of obese-diabetic (OBDC) rats treated with S. zalacca fruit using proton-nuclear magnetic resonance ( H NMR)-based metabolomics approach. This re search presents the first report on the in vitro antidiabetic effect of S. zalacca fruits ex tract us ing this approach. The obtained results in dicated that the administration of 400 mg/ kg bw of 60% ethanolic S. zalacca extract for 6 weeks significantly de creased the blood glucose level and normalized the blood lipid pro file of the OBDC rats. The potential biomarkers in urine were 2-oxoglutarate, alanine, leucine, succinate 3-hydroxy butyrate, taurine, betaine, allantoin, acetate, dimethylamine, creatine, creatinine, glucose, phenyl-acetyl glycine, and hippurate. Based on the data obtained, the metabolite pro files of the urine of treated rats by the 60% ethanolic extract could not be fully improved the metabolic complications of diabetic rats. The ex tract of S. zalacca fruit was able to de crease the ketones bodies as 3-hydroxy butyrate and acetoacetate. It has also improved energy metabolism, involving glucose, acetate, lactate, 2-hydroxy butyrate, 2-oxoglutarate, citrate, and succinate. More over, it decreased metabolites from gut microflora, including choline. This extract had significant effect on amino acid metabolism, metabolites from gut microflora, bile acid metabolism and creatine. The result can further support the traditional claims of S. zalacca fruits in management of diabetes. This finding might bevaluable in understanding the molecular mechanism and pharmacological properties of this medicinal plant for managing diabetes mellitus.

Item Type: Article (Journal)
Additional Information: 6804/81676
Uncontrolled Keywords: Salacca zalacca; H NMR-based metabolomics; Diabetes; Metabolic disorders; Multivariate data analysis
Subjects: Q Science > QD Chemistry
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Pharmacy
Kulliyyah of Pharmacy > Department of Pharmaceutical Chemistry
Kulliyyah of Pharmacy > Department of Pharmacy Practice
Depositing User: Dr M Jamshed Siddiqui
Date Deposited: 17 Jul 2020 14:41
Last Modified: 17 Nov 2020 11:17
URI: http://irep.iium.edu.my/id/eprint/81676

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