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Antinociceptive activity of asiaticoside in mouse models of induced nociception

Ayumi, Rasyidah Ryta and Shaik Mossadeq, Wan Mastura and Zakaria, Zainul Amiruddin and Bakhtiar, M. Taher and Kamarudin, Nadhirah and Hisamuddin, Nadia and Talib, Madihah and Sabar, Aina Mardhiah (2020) Antinociceptive activity of asiaticoside in mouse models of induced nociception. Planta Medica. ISSN 0032-0943 (In Press)

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Abstract

The antinociceptive property of Centella asiatica extracts is known but the analgesic activity of its bioactive constituent asiaticoside has not been reported. We evaluated the antino- ciceptive activity of orally (p.o.) administered asiaticoside (1, 3, 5, and 10mg/kg) in mice using the 0.6% acetic acid-in- duced writhing test, the 2.5% formalin-induced paw licking test, and the hot plate test. The capsaicin- and glutamate-in- duced paw licking tests were employed to evaluate the in- volvement of the vanilloid and glutamatergic systems, respec- tively. Asiaticoside (3, 5, and 10 mg/kg, p. o.) reduced the rate of writhing (p < 0.0001) by 25.3, 47.8, and 53.9 %, respective- ly, and increased the latency period (p < 0.05) on the hot plate at 60 min post-treatment until the end of the experiment. Moreover, asiaticoside (3, 5, and 10 mg/kg, p. o.) shortened the time spent in licking/biting the injected paw (p < 0.0001) in the early phase of the formalin test by 45.7, 51.4, and 52.7 %, respectively, and in the late phase (p < 0.01) by 23.6, 40.5, and 50.6 %, respectively. Antinociception induced by asi- aticoside (10 mg/kg) was not antagonized by naloxone in both the 2.5 % formalin-induced nociception and the hot plate test, indicating a nonparticipation of the opioidergic system. Asia- ticoside (1, 3, 5, and 10 mg/kg, p. o.) reduced the duration of biting/licking the capsaicin-injected paw (p < 0.0001) by 40.5, 48.2, 59.5, and 63.5%, respectively. Moreover, asiaticoside (5 and 10 mg/kg) shortened the time spent in biting/licking the glutamate-injected paw (p < 0.01) by 29.9 and 48.6 %, re- spectively. Therefore, asiaticoside (5 and 10 mg/kg, p. o.) in- duces antinociception possibly through the vanilloid and glu- tamatergic systems.

Item Type: Article (Journal)
Additional Information: 5209/80138
Uncontrolled Keywords: Apiaceae, Centella asiatica, antinociception, mouse
Subjects: R Medicine > RS Pharmacy and materia medica
R Medicine > RS Pharmacy and materia medica > RS192 Materia Medica-Pharmaceutical Technology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Pharmacy
Kulliyyah of Pharmacy > Department of Pharmaceutical Technology
Depositing User: Dr Muhammad Taher
Date Deposited: 24 Apr 2020 10:14
Last Modified: 10 Jul 2020 16:00
URI: http://irep.iium.edu.my/id/eprint/80138

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