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Differential expression of miR-101 and miR-744 in nasopharyngeal carcinoma in Pahang state of Malaysia

Ahmad, Azmir and Ab. Wahid, Haziq and Leman, Wan Ishlah and Othman, Noor Syamila and Abd Rahman, Azlina and Abdullah, Kahairi and Muhammad, Siti Aesah @ Naznin and Kaderi, Mohd. Arifin (2019) Differential expression of miR-101 and miR-744 in nasopharyngeal carcinoma in Pahang state of Malaysia. Walailak Journal of Science and Technology, 16 (5). pp. 295-306. ISSN 1686-3933 E-ISSN 2228-835X

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Abstract

Previous study found that microRNA-101 (miR-101) and microRNA-744 (miR-744) were deregulated in head and neck cancers and were implicated in nasopharyngeal carcinoma (NPC) carcinogenesis. Thus, this study aimed to determine the expression of miR-101 and miR-744 in NPC and analyse the utility of these microRNAs (miRNAs) as diagnostic biomarkers. Total RNA was extracted from 31 NPC and 7 non-NPC control formalin-fixed paraffin-embedded (FFPE) samples. Complementary DNA (cDNA) was synthesized from the total RNA and proceeded with quantitative real-time polymerase chain reaction. Differential expression of miR-101 and miR-744 were calculated from quantification cycle (Cq) data using 2-ΔΔCq calculation. The performance of these miRNAs were calculated using receiver operating characteristic (ROC) curve analysis. The differential expression for miR-101 and miR744 were -1.39 (p < 0.05) and 2.48 (p > 0.05), respectively, where the deregulations were consistent with the previous report. The area under curve for miR-101, miR-744 and combination of miR-101 and miR744 were 0.654 (95 % CI: 0.465 - 0.844), 0.588 (95 % CI: 0.368 - 0.808) and 0.626 (95 % CI: 0.481 - 0.771), respectively. However, re-analysis using balanced sample size between NPC and non-NPC control group showed the value decreased to 0.653 (95 % CI: 0.347 - 0.959) for miR-101 but increased to 0.827 (95 % CI: 0.601 - 1.000) for miR-744 and 0.758 (95 % CI: 0.576 - 0.939) for the combination of miR-101 and miR-744, indicating the importance of having a balanced sample size. We have successfully determined the expression of miR-101 and miR-744 in NPC samples. We also demonstrated statistically the utility of these miRNAs as diagnostic biomarkers.

Item Type: Article (Journal)
Additional Information: 3643/79822
Uncontrolled Keywords: Nasopharyngeal carcinoma; miR-101, miR-744; Differential expression; Diagnostic biomarker
Subjects: R Medicine > R Medicine (General)
R Medicine > RC Internal medicine
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Allied Health Sciences > Department of Biomedical Science (Effective:1st July 2011)
Kulliyyah of Medicine > Department of Otolaryngology - Head and Neck Surgery
Kulliyyah of Medicine > Department of Pathology & Lab Medicine
Depositing User: Dr Kahairi Abdullah
Date Deposited: 25 Jun 2020 12:52
Last Modified: 25 Jun 2020 12:52
URI: http://irep.iium.edu.my/id/eprint/79822

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