Cytotoxic xanthones isolated from calophyllum depressinervosum and calophyllum buxifolium with antioxidant and cytotoxic activities

Zamakshshari, Nor Hisam and Ee, Gwendoline Cheng Lian and Ismail, Intan Safinar and Ibrahim, Zalikha and Mah, Siau Hui (2019) Cytotoxic xanthones isolated from calophyllum depressinervosum and calophyllum buxifolium with antioxidant and cytotoxic activities. Food and Chemical Toxicology, 133. ISSN 0278-6915 E-ISSN 873-6351

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Abstract

The stem bark of Calophyllum depressinervosum and Calophyllum buxifolium were extracted and examined for their antioxidant activities, together with cytotoxicity towards human cancer cells. The methanol extract of C. depressinervosum exhibited good DPPH and NO scavenging effects. The strongest BCB inhibition and FIC effects were shown by dichloromethane and ethyl acetate extracts of both species. Overall, DPPH, FRAP and FIC assays showed strong correlation with TPC. For cytotoxicity, hexane extract of C. depressinervosum possessed the strongest anti-proliferative activities towards SNU-1 cells while the hexane extract of C. buxifolium showed the strongest activity towards LS-174T and K562 cells with the IC values ranging from 7 to 17 μg/mL. The purification of plant extracts afforded eight xanthones, ananixanthone (1), caloxanthone B (2), caloxanthone I (3), caloxanthone J (4) xanthochymone B (5), thwaitesixanthone (6), 1,3,5,6-tetrahydroxyxanthone (7) and dombakinaxanthone (8). All the xanthones, except 1 were reported for the first time from both Calophyllum species. The xanthones were examined for their cytotoxic effect against K562 leukemic cells. Compounds 1 and 2 showed strong cytotoxicity with the IC values of 2.96 and 1.23 μg/mL, respectively. The molecular binding interaction of 2 was further investigated by performing molecular docking study with promising protein receptor Src kinase.

Item Type:	Article (Journal)
Additional Information:	7854/79802
Uncontrolled Keywords:	Caloxanthone B; K562 cells; Molecular docking; Radical scavenging activities
Subjects:	Q Science > QR Microbiology R Medicine > RC Internal medicine S Agriculture > SB Plant culture
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Pharmacy Kulliyyah of Pharmacy > Department of Pharmaceutical Chemistry
Depositing User:	Asst. Dr. Zalikha Ibrahim
Date Deposited:	24 Jun 2020 17:03
Last Modified:	24 Jun 2020 17:03
URI:	http://irep.iium.edu.my/id/eprint/79802

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