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The use of FTIR and Raman spectroscopy in combination with chemometrics for analysis of biomolecules in biomedical fluids: a review

,, Abdul Rohman and Windarsih, Anjar and Lukitaningsih, Endang and ,, Mohamad Rafi and Muflih, Betania Kartika and Ahmad Fadzillah, Nurrulhidayah (2019) The use of FTIR and Raman spectroscopy in combination with chemometrics for analysis of biomolecules in biomedical fluids: a review. Biomedical Spectroscopy and Imaging, 8 (3-4). pp. 55-71. ISSN 2212-8794

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Fourier transform infrared (FTIR) and Raman spectroscopy are complementary techniques, typically called vibrational spectroscopy. Both techniques allow simple, rapid, non-destructive, specific, providing fingerprint spectra, and real-time analytical method for analysis of molecules in different states. Besides, these methods are simple without any excessive sample pre-treatment, therefore, they are sometimes called as “green analytical methods”. Biofluids have several biomolecules such as lipid, protein, nucleic acids, and carbohydrates. These biomolecules can be used as biomarkers to detect some types of diseases, since biomolecules are in direct contact with the human organs. FTIR and Raman spectra of biofluids are complex in nature, therefore sophisticated statistical techniques, known as chemometrics, must be used to solve the analytical problems related to quantitative analysis purposes. The objective of this review is to show the capability of FTIR and Raman spectroscopic techniques in combination with chemometrics techniques to analyze the biomolecules in biofluids through an extensive literature review. During performing this review, several databases in Science citation index, Scopus PubMed, and Google Scholar related to the topics are identified and downloaded. With the present review, it is known that FTIR and Raman techniques are rapid method for screening certain diseases by identifying the level changes of some biomolecules. In the future, this method will be widely used for clinicians as new diagnostic tools for many diseases.

Item Type: Article (Review)
Additional Information: 7251/79421
Uncontrolled Keywords: FTIR spectroscopy, Raman spectroscopy, chemometrics, biomolecules, biofluids
Subjects: Q Science > Q Science (General)
Q Science > QD Chemistry
Q Science > QP Physiology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): International Institute for Halal Research and Training (INHART)
Depositing User: mrs betania muflih
Date Deposited: 05 Mar 2020 10:06
Last Modified: 05 Mar 2020 10:06
URI: http://irep.iium.edu.my/id/eprint/79421

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