Discrimination of clinacanthus nutans extracts and correlation with antiplasmodial activity using ATR-FTIR fingerprinting

Noor Hashim, Noor and Ali, Amatul Hamizah and Khatib, Alfi and Latip, Jalifah (2019) Discrimination of clinacanthus nutans extracts and correlation with antiplasmodial activity using ATR-FTIR fingerprinting. Vibrational Spectroscopy, 104. pp. 1-6. ISSN 092-42031

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Abstract

Clinacanthus nutans or Sabah Snake Grass is a small shrub indigenous to tropical Asia. It has been used in the treatment of skin rashes, insect and snake bites, cancer and diabetes. In this study, Fourier transform infrared spectroscopy was used to discriminate the effects of different extraction methods on the chemical composition of C. nutans freeze dried leaves and stems. The samples were extracted using different methods (soaking and sonication) followed by the measurement of its antiplasmodial activity. Based on the absorption peaks, C. nutans extracts comprise of compounds with hydroxyl, methyl, sulfoxide, sulfone, aromatic and carbonyl functionalities which indicate the presence of carbohydrates, terpenes, sulfurous glycosides and aromatic compounds in the extracts. Multivariate data analysis such as orthogonal partial least square (OPLS) was used to correlate the FTIR spectra of the extracts with antiplasmodial activity. The OPLS model exhibited R2Y and Q2Y values of 0.896 and 0.736, respectively. The RMSEE and RMSECV value were 22.41 and 32.36. The loading line plot of the OPLS model revealed that OH, C[dbnd]O, C[sbnd]H and C[sbnd]C functional group induced the activity, whereas, C[sbnd]N and S[dbnd]O reduced the bioactivity. These preliminary results demonstrated that FTIR spectroscopy can be used as a simple and rapid technique to discriminate C. nutans extracts obtained from different extraction methods which is useful in the quality control during processing of this plant.

Item Type:	Article (Journal)
Additional Information:	6915/79147
Uncontrolled Keywords:	Clinacanthus nutans, Fourier transform infrared spectroscopy (FTIR) Antiplasmodial, Metabolomics, Multivariate data analysis,
Subjects:	Q Science > Q Science (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Pharmacy Kulliyyah of Pharmacy > Department of Pharmaceutical Chemistry
Depositing User:	Dr. Alfi Khatib
Date Deposited:	26 Feb 2020 15:27
Last Modified:	29 Sep 2021 12:32
URI:	http://irep.iium.edu.my/id/eprint/79147

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