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Toxicity study on Clinacanthus nutans leaf hexane fraction using Danio rerio embryos

Murugesu, Suganya and Khatib, Alfi and Ahmed, Qamar Uddin and Ibrahim, Zalikha and Uzir, Bisha Fathamah and Benchoula, Khaled and Nik Yusoff, Nik Mohd. Idris and Perumal, Vikneswari and Alajmi, Mohamed Fahad and Salamah, Sahal and El-Seedi, Hesham (2019) Toxicity study on Clinacanthus nutans leaf hexane fraction using Danio rerio embryos. Toxicology Reports, 6. pp. 1148-1154. E-ISSN 2214-7500

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Clinacanthus nutans, an herbal shrub belonging to the Acanthaceae family, is traditionally used as a functional food to treat various ailments in Malaysia and Indonesia. Although the polar fraction of this plant shows nontoxic effect, the toxicity of the non-polar extract is not reported so far. The present study aimed to assess the toxic effect and determine the lethal concentration of this non-polar fraction using zebrafish embryos. The n-hexane fraction was partitioned from the crude extract of C. nutans obtained using 80% methanolic solution. After spawning of the adult male and female zebrafish, the eggs were collected, transferred into a 96-well plate and incubated with the n-hexane fraction at concentrations of 15.63 μg/ml, 31.25 μg/ml, 62.5 μg/ml, 125 μg/ml, 250 μg/ml and 500 μg/ml in 2% DMSO. The survival and sublethal endpoint were assessed, the mortality and hatchability rates were calculated based on microscopic observation, while the heartbeat rate was measured using DanioScope software. The median lethal concentration (LC50) of the C. nutans n-hexane fraction, which was determined using probit analysis, was calculated to be 75.49 μg/mL, which is harmful. Moreover, gas chromatography-mass spectrometry (GC–MS) analysis revealed the presence of palmitic acid, phytol, hexadecanoic acid, 1-monopalmitin, stigmast-5-ene, pentadecanoic acid, heptadecanoic acid, 1-linolenoylglycerol and stigmasterol in the n-hexane fraction.

Item Type: Article (Journal)
Additional Information: 6195/79118
Uncontrolled Keywords: Clinacanthus nutans, Danio rerio, DanioScope, Median lethal concentration, Teratogenicity Probit analysis
Subjects: R Medicine > R Medicine (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Pharmacy
Kulliyyah of Pharmacy > Department of Pharmaceutical Chemistry
Depositing User: Dr. Alfi Khatib
Date Deposited: 26 Feb 2020 11:44
Last Modified: 27 Mar 2020 21:40
URI: http://irep.iium.edu.my/id/eprint/79118

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