The effect of Pegagan ethanolic extract (Centella asiatica L) self-nanoemulsifying drug delivery system (SNEDDs) in the development of Zebrafish embryos (Danio rerio)

Hayati, Farida and Chabib, Lutfi and Fauzi, Silmi and Awaluddin, R and , Sumayya and Mohd Nasir, Mohd Hamzah and Faizah, Wan Syarifah (2019) The effect of Pegagan ethanolic extract (Centella asiatica L) self-nanoemulsifying drug delivery system (SNEDDs) in the development of Zebrafish embryos (Danio rerio). In: International Conference on Pharmaceutical Research and Pharmacy Practice cum 14th IIUM-MPS Pharmacy Scientific Conference (ICPRP 2019), 19th-20th October 2019, Kuala Lumpur. (Unpublished)

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Abstract

INTRODUCTION PegaganThe pegagan is a traditional medicinal plant with the three major bioactive properties, triterpenoid, steroids, and saponin. It has the properties of antioxidant, anti-stress, and wound healing. Pegagan extract is prepared was made in Self-Nano emulsifying Drug Delivery Systems (SNEDDS) to overcome the problem of pegagan extract which has a low water-solubility level on water. OBJECTIVES The aim of this study was to observed the effect of pegagan ethanolic extract SNEDDS in the development of zebrafish embryos (toxicity and teratogenicity are the term for Fish Embryo Toxicity (FET) as outlined by OECD). METHOD This study used 12 sets of zebrafish embryos which will be presented by five sets of extract SNEDDS extracts with different concentrations, i.e. 20μg, 10μg, 5μg, 2.5μg, and 1.25μg, five sets of SNEDDS without extract with different concentrations, i.e. 20μg, 10μg, 5μg, 2.5μg, and 1.25μg, a set of positive controls ((3.4-DCA 4mg / L) with one control set (diluted withdilution water), and a negative control (SNEDDS without extract). The procedure was conducted for 96 hours with observations every 24 hours. The parameters observed were embryonic coagulation, formation of somites, detachment of the tail-bud form part of the yolk, and pulse abnormality of embryo RESULTS The results showed that in 96 hours the 20 ppm concentration caused 100% highest mortality. Embryo abnormality that appeared as coagulation of embryo, somite malformation, and tail abnormal tail. DISCUSSION There is a correlation between concentration of SNEDDS and the incidence of embryo coagulation. The malformation in the group of PES is characterized by cardiac edema, somite malformation, and abnormal tail. CONCLUSION PES 20 ppm can affect the development of zebrafish embryos.

Item Type:	Conference or Workshop Item (Plenary Papers)
Additional Information:	5522/78177
Uncontrolled Keywords:	Centella asiatica L, SNEDDS, zebra fish, coagulation
Subjects:	Q Science > Q Science (General) Q Science > QH Natural history Q Science > QH Natural history > QH301 Biology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Science Kulliyyah of Science > Department of Biotechnology
Depositing User:	Dr Mohd Hamzah Mohd Nasir
Date Deposited:	06 Mar 2020 11:46
Last Modified:	06 Mar 2020 11:46
URI:	http://irep.iium.edu.my/id/eprint/78177

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