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Variations in neurotoxicity and proteome profile of Malayan krait (Bungarus candidus) venoms

Ahmad Rusmili, Muhamad Rusdi and Othman, Iekhsan and Zainal Abidin, Syafiq Asnawi and Yusof, Fatin Athirah and Ratanabatangkoon, Kavi and Chanhome, Lawan and Hodgson, Wayne C. and Chaisakul, Janeyut (2019) Variations in neurotoxicity and proteome profile of Malayan krait (Bungarus candidus) venoms. PLoS ONE, 14 (12). pp. 1-19. ISSN 1932-6203

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Malayan krait (Bungarus candidus) is a medically important snake species found in Southeast Asia. The neurotoxic effects of envenoming present as flaccid paralysis of skeletal muscles. It is unclear whether geographical variation in venom composition plays a significant role in the degree of clinical neurotoxicity. In this study, the effects of geographical variation on neurotoxicity and venom composition of B. candidus venoms from Indonesia, Malaysia and Thailand were examined. In the chick biventer cervicis nerve-muscle preparation, all venoms abolished indirect twitches and attenuated contractile responses to nicotinic receptor agonists, with venom from Indonesia displaying the most rapid neurotoxicity. A proteomic analysis indicated that three finger toxins (3FTx), phospholipase A2 (PLA2) and Kunitz-type serine protease inhibitors were common toxin groups in the venoms. In addition, venom from Thailand contained L-amino acid oxidase (LAAO), cysteine rich secretory protein (CRISP), thrombin-like enzyme (TLE) and snake venom metalloproteinase (SVMP). Short-chain post-synaptic neurotoxins were not detected in any of the venoms. The largest quantity of long-chain post-synaptic neurotoxins and non-conventional toxins was found in the venom from Thailand. Analysis of PLA2 activity did not show any correlation between the amount of PLA2 and the degree of neurotoxicity of the venoms. Our study shows that variation in venom composition is not limited to the degree of neurotoxicity. This investigation provides additional insights into the geographical differences in venom composition and provides information that could be used to improve the management of Malayan krait envenoming in Southeast Asia.

Item Type: Article (Journal)
Additional Information: 5591/77854
Uncontrolled Keywords: BFAV, B. fasciatus monovalent antivenom; BCAV, B. candidus monovalent antivenom; BC-T, Bungarus candidus from Southern Thailand; BC-I, Bungarus candidus from Indonesia; BC-M, Bungarus candidus from Peninsular Malaysia; Ach, Acetylcholine; CCh, carbachol; KCl, potassium chloride; 3FTx, three finger toxins; PLA2, phospholipase A2; LAAO, Lamino acid oxidase; CRISP, cysteine rich secretory protein; TLE, thrombin-like enzyme; SVMP, snake venom metalloproteinase; HPLC, High Performance Liquid Chromatography; LCMS, Liquid Chromatography Mass Spectrometry.
Subjects: Q Science > QH Natural history
Q Science > QL Zoology
R Medicine > RM Therapeutics. Pharmacology
R Medicine > RM Therapeutics. Pharmacology > RM300 Drugs and their action
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Pharmacy
Kulliyyah of Pharmacy > Department of Basic Medical Sciences
Depositing User: Dr Muhamad Rusdi Ahmad Rusmili
Date Deposited: 09 Jan 2020 14:23
Last Modified: 14 Apr 2020 15:59
URI: http://irep.iium.edu.my/id/eprint/77854

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