Hydrodistillation and soxhlet extraction of agarwood leaf extract from Aquilaria malaccensis

Hashim, Yumi Zuhanis Has-Yun and Mat Jamil, Mohamad Akmal and Jamal, Parveen and Zainurin, Nur Aimi Aliah and Syed Abdul Azzizz, Saripah Salbiah (2019) Hydrodistillation and soxhlet extraction of agarwood leaf extract from Aquilaria malaccensis. Malaysian Journal of Fundamental and Applied Science, 15 (6). 842 -846. ISSN 2289-5981 E-ISSN 2289-599X.

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Abstract

Agarwood (A. malaccensis) is a valuable tree; highly sought after the resin and its essential oil. It also possesses bioactive compounds that are beneficial for health. However, less is focused on the agarwood leaf despite the recorded use in traditional medicine. As such, this present work focused on the screening of operational parameters of hydrodistillation and Soxhlet extraction of agarwood leaf extract (ALEX) with subsequent phytochemical and gas chromatography mass spectrometry (GCMS) analyses. Hydrodistillation failed to obtain any essential oil. Therefore, only soxhlet extraction was further studied based on Plackett-Burman design generated using design expert software, version 6. The parameters studied were solvent type and volume, time, leaf type and particle size. Based on the ALEX yield obtained, solvent type was found to be the most significant parameters followed by solvent volume, particle size and time. Meanwhile leaf type was found to be the least influential parameter. Ethanol gave higher yield as compared to hexane. Run 2 gave the highest ALEX yield (121.34 ± 31.6 mg/g) and Run 4 gave the lowest yield (20.38 ± 4.1 mg/g). Based on phytochemical analysis, ALEX possess phenol, flavonoid, alkaloid, saponin and steroid compounds . GCMS analysis has identified a total of 50 compounds from ALEX in 12 experiments. Hexadecanoic acid was found to be the major compound in run 2 (highest yield) and phytol was found to be the major compound in run 4 (lowest yield). To this end, ALEX was succesfully obtained through Soxhlet extraction and the significant parameters can be further studied to achive optimal yield.

Item Type:	Article (Journal)
Additional Information:	3774/77203
Uncontrolled Keywords:	Agarwood, hydrodistillation; Operational parameters; Phytochemicals; Soxhlet
Subjects:	Q Science > Q Science (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	International Institute for Halal Research and Training (INHART)
Depositing User:	DR YUMI ZUHANIS HAS-YUN HASHIM
Date Deposited:	09 Jan 2020 11:39
Last Modified:	09 Jan 2020 11:39
URI:	http://irep.iium.edu.my/id/eprint/77203

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