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The effects of progesterone administration in mice during pregnancy on ovarian development and anogenital distance of the offspring

Mohammad, Najat A. and Selman, Mohammad O. and Al-Bakri, Nahla and Matloupe, Enas I. and Al-Ani, Imad Matloub Dally and Faisal, Ghasak Ghazi and Suhail, Azhar M. (2019) The effects of progesterone administration in mice during pregnancy on ovarian development and anogenital distance of the offspring. Journal of International Dental and Medical Research, 12 (4). pp. 1665-1670. ISSN 1309-100x

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Background: Progesterone is highly used in pregnant women as therapeutic agent to maintain and support pregnancy. Objective: To explore the effects of progesterone usage allover gestation till 7days postnatally on mice offspring ovaries development and anogenital distance. Material and methods: Ten pregnant mice were equally divide into control group that was injected with sesame oil which is used as a solvent for progesterone and treated group that is daily intraperitoneally injected with progesterone (dissolved in sesame oil 1:10) at dose 10.2mg/kg (the equivalent human dose) all through gestation till7day postnatal then sacrificed and measuring the anogenital distance (the distance between anus and genital papilla). Histological slides were prepared and Diameters of the ovary, primary oocyte and primordial follicles were measured and histopathological changes analysis was done. Result: Progesterone administration cause significant increment (p>0-05) in anogenital distance, significant decrement in primary oocyte diameter and primordial follicle diameter, with no significant difference in the ovary diameter. Histopathological changes were seen as hemorrhage, detachment of follicular cells from basement membrane with irregular arrangement and thickening or death of follicular cells, pyknosis of primary oocytes and vacculation. Stromal cells degeneration. Conclusion: The current study revealed that progesterone injection of mice with equivalent human dose during pregnancy is embryotoxic and teratogenic, may alter the female reproductive performance with virilizing the female genitalia. The benefit of progesterone as a therapies need to be proven before recommended as supportive treatment during pregnancy.

Item Type: Article (Journal)
Additional Information: 6388/76815
Uncontrolled Keywords: progesterone, ovaries, mice offspring, anogenital distance, virilization
Subjects: R Medicine > RB Pathology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Dentistry
Kulliyyah of Dentistry > Department of Fundamental Dental and Medical Sciences
Kulliyyah of Medicine
Kulliyyah of Medicine > Department of Basic Medical
Depositing User: Assoc.Prof Ghasak Ghazi Al-Atrakchi
Date Deposited: 04 Dec 2019 16:16
Last Modified: 19 Aug 2020 10:35
URI: http://irep.iium.edu.my/id/eprint/76815

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