Fitri, Muhammad and Siraj, Siti Shapor and Nakajima, Masamichi (2015) Cross-species amplification of microsatellite DNA markers and comparison on the sequence of microsatellite regions among Southeast Asian Catfish species. Fish Genetics and Breeding Science, 43 (2). pp. 49-57. ISSN 1343-7917
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Abstract
Thirty microsatellite markers developed for River catfish (Hemibagrus nemurus) were screened for cross amplification in six species of catfish. Out of 30 primers, only 3 (10) to 6 (20) produced successful amplifications in six catfish species. Six different loci could be amplified in Pangasius pangasius and Clarias batrachus, 5 loci in Clarias gariepinus, 4 loci in Pseudomystus siamensis and 3 loci in Clarias macrocephalus and Pangasius nasutus. The low rate of amplification in these 6 closely related species proved the primers are locus-specific. Sequence analysis of locus MnRm7-1 showed differences in the flanking region and repeat motif in certain species, suggesting the existence of indel mutations in catfish species in that particular region. The examined DNA sequence revealed that the repeat motif is conser ved within Pangasiid catfishes and not conser ved within Clariid catfishes
| Item Type: | Article (Journal) |
|---|---|
| Additional Information: | 7829/76559 |
| Uncontrolled Keywords: | microsatellite markers; catfish; aquaculture; population genetics |
| Subjects: | S Agriculture > SH Aquaculture. Fisheries. Angling S Agriculture > SH Aquaculture. Fisheries. Angling > SH151 Aquaculture - Fish Culture |
| Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): | Kulliyyah of Science > Institute of Oceanography and Maritime Studies |
| Depositing User: | Dr Fitri Yusof |
| Date Deposited: | 26 Dec 2019 16:34 |
| Last Modified: | 26 Dec 2019 16:35 |
| URI: | http://irep.iium.edu.my/id/eprint/76559 |
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