Identification of hyaluric acid synthesis 2 (has2) and gremlin 1 (grem1) gene expressions in human cumulus cells as a biomarker for oocyte quality.

Abdul Wahab, Azantee Yazmie and Md Isa, Muhammad Lokman and Mohd Yassim, Haiyuni and Salleh, Yukmil Amru and Mat Yusof, Afzan and Abdul Razak, Redzuan Nul Hakim and Abdul Razak, Nik Fakhuruddin and Muhammad, Hussin and Mazlan, Muhammad Alif (2019) Identification of hyaluric acid synthesis 2 (has2) and gremlin 1 (grem1) gene expressions in human cumulus cells as a biomarker for oocyte quality. Malaysian Journal of Biochemstry and Molecular Biology, 22 (2). pp. 41-45. E-ISSN 2600-9005

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Abstract

Nowadays, the criteria for oocyte selection is based on morphological criteria however, it needs further improvement to select the best embryo quality. The gene expression in the cumulus cell plays important role in signaling for follicular development as well as for oocyte quality. The aim of this study is to investigate the present of HAS2 and GREM1 gene expression in the cumulus cells that can become a useful marker for oocyte quality. A preliminary study was performed on cumulus cells derived from four different patients that undergo assisted reproductive technique treatment. Cumulus cells were isolated from 4 patients and the expression of HAS2 and GREM1 was analyzed by using reverse transcriptase polymerase chain reaction (rt- PCR). The results shown that HAS2 and GREM1 were expressed in grade 3 oocytes, whereas, the genes were absent in grade 4 oocytes. This showed that the expression influenced the oocyte quality. Hence, the measurement of HAS2 and GREM1 expressions in cumulus cells would reliably useful tool for selecting competence oocytes with greater chances to be fertilized in assisted reproductive technique.

Item Type:	Article (Review)
Additional Information:	4508/76226
Uncontrolled Keywords:	Cumulus cells, HAS2, GREM1, rt-PCR, oocyte quality
Subjects:	R Medicine > R Medicine (General) R Medicine > RG Gynecology and obstetrics R Medicine > RT Nursing
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Allied Health Sciences > Department of Biomedical Science (Effective:1st July 2011) Kulliyyah of Medicine > Department of Obstetric & Gynecology Kulliyyah of Nursing > Department of Basic Medical Sciences for Nursing
Depositing User:	salina mohammad
Date Deposited:	16 Dec 2019 18:27
Last Modified:	24 Mar 2020 18:19
URI:	http://irep.iium.edu.my/id/eprint/76226

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