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Structural integrity of aortic scaffolds decellularized by sonication decellularization system

, Aqilah Hazwani and Sha’ban, Munirah and Azhim, Azran (2019) Structural integrity of aortic scaffolds decellularized by sonication decellularization system. In: "2018 IEEE EMBS Conference on Biomedical Engineering and Sciences, IECBES 2018", 3 - 6 December 2018, Borneo Convention Centre KuchingDemak-Isthmus Bridge, Sejingkat Kuching, Sarawak.

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Abstract

Sonication decellularization technique has shown effectiveness to remove all the cellular components by the disruption of the cell membranes and removal of the cell debris to prepare the bioscaffolds. However, it is important to confirm whether this technique does not have a detrimental effect on elastin and collagen in bioscaffolds. The objectives of this study are to evaluate the structural integrity of bioscaffolds using histological staining and quantitatively collagen and elastin measurement. Aortic tissues were sonicated in 0.1% SDS for 10 hours at the frequency of 170 kHz with the power output of 15W and washed in Phosphate Buffer Solution (PBS) for 5 days. Then the sonicated aortic tissues were evaluated by Hematoxylin & Eosin (H&E) staining for cell removal analysis, Verhoeff-van Gieson (VVG) staining for visualizing elastin and Picrosirius Red (PSR) staining for visualizing collagen. The collagen and elastic fibres were semi-quantified by ImageJ software. The results showed that sonication decellularization system can remove all the cellular components while maintaining the structural integrity of elastin and collagen on bioscaffolds. This study indicates that sonication decellularization system could remove all cellular components and maintain the structure of the extracellular matrix.

Item Type: Conference or Workshop Item (Plenary Papers)
Additional Information: 6006/76211
Uncontrolled Keywords: Sonication; Decellularization; Bioscaffolds; Aorta; Elastin, collagen.
Subjects: Q Science > Q Science (General)
Q Science > QR Microbiology
R Medicine > R Medicine (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Allied Health Sciences
Kulliyyah of Allied Health Sciences > Department of Biomedical Science (Effective:1st July 2011)
Depositing User: Munirah Sha'ban
Date Deposited: 13 Nov 2019 21:42
Last Modified: 13 Nov 2019 21:42
URI: http://irep.iium.edu.my/id/eprint/76211

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