Phenolic profiling of selected herbal plants: free, soluble bound and insoluble bound phenolics and their antioxidant activities

Mohd Yusof, Nor Amaliana Syazwani and Haron, Normah and Mat Jusoh, Hanapi (2019) Phenolic profiling of selected herbal plants: free, soluble bound and insoluble bound phenolics and their antioxidant activities. Advances in Bioresearch, 10 (1). pp. 9-15. ISSN 0976-4585 E-ISSN 2277-1573

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Abstract

This study was conducted to profile selected phenolic acids, total phenolic content and antioxidant activities of five herbal plants namely Citrus hystrix, Coriandrum sativum, Murraya koenigii, Polygonum minus and ocimum basilicum in three different phenolic fractions (free, soluble bound and insoluble bound). The phenolic content was quantified using Folin-Ciocalteu method and phenolic acids were separated using C6-phenyl column High Performance Liquid chromatography. The antioxidant properties were determined using DPPH (2, 2-Diphenyl-1-picrylhydrazyl) and ABTS (2,2’-Azino-bis (3-ethylbenzothiazoline-6-sulphonic acid)) assays. The result showed that gallic, caffeic, ferulic, sinapinic and p-coumaric acids were detected in distinctive proportion. Bound forms of phenolic acids (soluble and insoluble bound) have high phenolic contents as compared to free phenolic. P. minus and O. basilicum demonstrated higher total phenolic content and showed promising antioxidant activities .The antioxidant properties can be correlated with the presence of phenolic acids in the extracts. To conclude, the herbal plants have the potential in reducing ability due to their strong electron-donating effects.

Item Type:	Article (Journal)
Additional Information:	7032/76003
Uncontrolled Keywords:	Free phenolic, bound phenolic, phenolic acids, herbal plants, antioxidant, free radical
Subjects:	Q Science > Q Science (General) Q Science > QK Botany S Agriculture > S Agriculture (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Allied Health Sciences > Department of Nutrition Sciences Kulliyyah of Science > Department of Biotechnology
Depositing User:	Dr. Normah Haron
Date Deposited:	05 Dec 2019 18:38
Last Modified:	05 Dec 2019 18:38
URI:	http://irep.iium.edu.my/id/eprint/76003

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