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Analysis on the inhibition of periodontal pathogens and its virulence genes expression by virgin coconut oil using quantitative real-time reverse transcription-PCR (qRT-PCR)

Abllah, Zurainie and Omar, Muhammad Nor and Arief Ichwan, Solachuddin Jauhari and Shahdan, Intan Azura and Jalaludin, Anil Azura and Haron, Ummi Aqilah and Mukhtar, Nor Izzah (2019) Analysis on the inhibition of periodontal pathogens and its virulence genes expression by virgin coconut oil using quantitative real-time reverse transcription-PCR (qRT-PCR). Project Report. UNSPECIFIED. (Unpublished)

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Abstract

Introduction: Dental caries has remained a major oral health problem worldwide. Streptococcus mutans is considered as a vital cariogenic agent in the oral cavity. It can synthesise soluble and insoluble glucans from sucrose by glucosyltransferases enzymes and develops stable biofilm on the tooth surface. In the previous study, the fatty acids profile of activated virgin coconut oil (AVCO) have been described, and it includes the excellent antimicrobial activity of the oil. The current study aims to evaluate the antibiofilm effect of AVCO against the Streptococcus mutans ATCC 2157 biofilm in vitro. Materials and method: The minimum biofilm inhibition concentration (MBIC) and the minimum biofilm eradication concentration (MBEC) for antibiofilm activity were determined by serial dilution method, and biofilm thickness on S. mutans was quantified by confocal laser scanning microscopy (CLSM). Results: At the concentra-tion of 15.63 mg/ml of AVCO, it successfully inhibits the development of S. mutans ATCC 21575 biofilm and AVCO (62.52 mg/ml) eradicate biofilm that was formed by the bacteria. The z-stack images obtained from CLSM allows the construction of 3-D biofilm structure and a significant difference in the thickness of S. mutans ATCC 21575 biofilm pre and post-treatment with AVCO were observed. Conclusion: AVCO showed good potential as anticaries where it possesses the ability to inhibits and eradicate S. mutans ATCC 21575 biofilm. A future study to evaluates the interaction of individuals fatty acids present in AVCO against proteins that relate to biofilm formation of S. mutans can be performed utilising the molecular docking tools.

Item Type: Monograph (Project Report)
Additional Information: 5620/75362
Uncontrolled Keywords: virgin coconut oil,vco,caries
Subjects: R Medicine > RK Dentistry > RK318 Oral and Dental Medicine. Pathology. Diseases-Therapeutics-General Works
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Dentistry > Paediatric Dentistry, Orthodontics & Dental Public Health
Kulliyyah of Allied Health Sciences
Kulliyyah of Science
Depositing User: Dr zurainie abllah
Date Deposited: 15 Oct 2019 16:30
Last Modified: 15 Oct 2019 16:30
URI: http://irep.iium.edu.my/id/eprint/75362

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