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In vivo anxiolytic and in vitro anti-inflammatory activities of water-soluble extract (WSE) of Nigella sativa (L.) seeds

Mohammed Babar, Zahir Uddin and Jaswir, Irwandi and Tareq, Abu Montakim and Ali Reza, A. S. M. and Wan Sulaiman, Wan Mohd Azizi and Mahamad Maifiah, Mohd Hafidz and Ahfter, F. and Hasan, Md Imran and Farhad, Saif and Uddin, Md. Rokib and Ichwan, Solachuddin J. A. and Ahmed, Qamar Uddin and Bakhtiar, M. Taher and Mawa, Imranul (2019) In vivo anxiolytic and in vitro anti-inflammatory activities of water-soluble extract (WSE) of Nigella sativa (L.) seeds. Natural Product Research. pp. 1-6. ISSN 1478-6419 E-ISSN 1478-6427

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Abstract

The WSE is a highly polar, gummy and mucilaginous bioactive content of the Nigella sativa (L.) seeds. This study reports the anxiolytic and anti-inflammatory effects of WSE investigated using Elevated Plus Maze (EPM) and Hole-Board Test (HBT) in adult mice and human RBCs haemolysis inhibition and protein denaturation respectively. The oral WSE treatment (100 & 200 mg/kg b.w/day) for 72 hours has exhibited slightly better anxiolytic effect (p < 0.05) through the time span (92.33 & 93.33 s) spent in the opened arms of EPM vs. diazepam (1 mg/kg b.w i.p/day; 69.33 s). In HBT, only WSE (200 mg/kg b.w/day) has shown a promising number of mean head pokes (13.27 times/min) vs. diazepam (12.87 times/min). The WSE (62.5-500 mg/mL) exposure has exhibited 40.14-72.18% protection against lysis of RBCs vs. aspirin (57.04-71.48%) whilst 62.67-67.66% inhibition of protein denaturation vs. diclofenac sodium (43.11-80.64%). The current findings suggested WSE has promising anxiolytic and anti-inflammatory activities.

Item Type: Article (Journal)
Additional Information: 5784/75208
Uncontrolled Keywords: Nigella sativa (L.) seeds; WSE; elevated plus maze (EPM); hole-board test (HBT); RBC haemolysis; anxiolytic; anti-inflammatory
Subjects: Q Science > QD Chemistry
R Medicine > RS Pharmacy and materia medica > RS403 Materia Medica-Pharmaceutical Chemistry
S Agriculture > S Agriculture (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): International Institute for Halal Research and Training (INHART)
Kulliyyah of Dentistry
Kulliyyah of Pharmacy
Kulliyyah of Pharmacy > Department of Pharmaceutical Chemistry
Kulliyyah of Pharmacy > Department of Pharmaceutical Technology
Depositing User: Dr Mohd Hafidz Mahamad Maifiah
Date Deposited: 08 Jan 2020 16:28
Last Modified: 08 Jan 2020 16:28
URI: http://irep.iium.edu.my/id/eprint/75208

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