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Application of direct fluorescence-based live/dead staining for assessment of antifungal activity of coconut oil against candida albicans

Mukhtar, Nor Izzah and Abllah, Zurainie and M., Azrul Naim and Shahdan, Intan Azura and Long, Kamariah and Haron, Ummi Aqilah (2019) Application of direct fluorescence-based live/dead staining for assessment of antifungal activity of coconut oil against candida albicans. Malaysian Journal of Analytical Sciences, 23 (5). pp. 1-6. ISSN 1394-2506 (In Press)

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Abstract

Candida albicans is one of the common causes for oral candidiasis worldwide. The proliferation of antifungal-resistant C. albicans has become a major concern. This study was carried out to evaluate activated virgin coconut oil (AVCO) and crude extract of virgin coconut oil (VCO) as new antifungal agents for treatment of oral candidiasis. C. albicans viability was determined using LIVE/DEAD bacterial viability kit. C. albicans cells were grown in yeast peptone dextrose (YPD) broth overnight. The fungus was treated with AVCO and VCO at the concentration of minimum fungicidal concentration (MFC) of 6.24 mg/mL and incubated for three different time points (1, 2, and 3 h). To evaluate the viability of C. albicans, SYTO 9 and propidium iodide (PI) staining were used and the cells were observed using fluorescence microscopy. C. albicans treated with AVCO showed more dead cells compared to cells treated with VCO. The data indicate that exposure of C. albicans to AVCO was the most inhibitory to its growth (p < 0.01).

Item Type: Article (Journal)
Additional Information: 5620/75103
Uncontrolled Keywords: LIVE/DEAD staining, `antifungal activity, Candida albicans, virgin coconut oil
Subjects: R Medicine > RK Dentistry > RK318 Oral and Dental Medicine. Pathology. Diseases-Therapeutics-General Works
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Allied Health Sciences
Kulliyyah of Dentistry > Paediatric Dentistry, Orthodontics & Dental Public Health
Kulliyyah of Science
Depositing User: Dr zurainie abllah
Date Deposited: 10 Oct 2019 16:40
Last Modified: 20 Jan 2020 16:45
URI: http://irep.iium.edu.my/id/eprint/75103

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