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Structural and functional properties of neocartilage construct engineered in poly (Lactic-co-Glycolic Acid) (PLGA) based scaffolds

Md Ali @ Tahir, Aisyah Hanani and Mohamed Amin, Muhammad Azri Ifwat and Noor Azmi, Azran Azhim and Sha'ban, Munirah (2019) Structural and functional properties of neocartilage construct engineered in poly (Lactic-co-Glycolic Acid) (PLGA) based scaffolds. International Journal of Integrated Engineering, 11 (3). pp. 109-118. ISSN 2229-838X E-ISSN 2600-7916

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Abstract

The study aims to assess the structural and functional properties of in vitro three-dimensional (3D) PLGA-based hybrid scaffolds seeded with chondrocytes, particularly in terms of the production of specific cartilaginous extracellular matrix (ECM). The PLGA scaffolds were incorporated with atelocollagen and/or fresh fibrin and assigned to four groups; PLGA only as control, PLGA-fibrin (PF), PLGA-atelocollagen (PA) and PLGA-atelocollagen-fibrin (PAF). The resulting PLGA hybrid scaffolds were characterized based on gross appearance, attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy analysis, porosity and swelling tests as well as cytocompatibility analysis using cell proliferation (MTT) assay. All scaffolds seeded with cells were cultured for three weeks in vitro. Macroscopic changes were recorded using photographs. Microscopic evaluation of ‘cells-scaffolds’ construct was done using Haematoxylin and Eosin (H&E), Safranin O, Alcian Blue, Toluidine Blue and scanning electron microscopy (SEM). The production of cartilage specific ECM was measured using sulphated glycosaminoglycan (sGAG) assay. Based on physical characterizations, PLGAbased hybrid scaffolds have been successfully manufactured and showed no cytocompatibility issues. The PAF exhibited cartilaginous tissue morphology better than other scaffold groups, grossly and microscopically. On SEM, the presence of branching fibers that produce a web-like network on the surface of PLGA-based hybrid scaffolds indicated ECM secretion. This is supported by the manifestation of glycosaminoglycan and as well as proteoglycan through histology and sGAG assay. This present study indicated that PLGA-based hybrid scaffolds promote formation of neocartilage in vitro.

Item Type: Article (Journal)
Additional Information: 7380/74944
Uncontrolled Keywords: tissue engineering, neocartilage, extracellular matrix, bioscaffold, PLGA, atelocollagen, fibrin
Subjects: T Technology > TA Engineering (General). Civil engineering (General) > TA164 Bioengineering
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Allied Health Sciences > Department of Biomedical Science (Effective:1st July 2011)
Kulliyyah of Allied Health Sciences
Depositing User: Ir Dr Azran Azhim Noor Azmi
Date Deposited: 24 Oct 2019 10:58
Last Modified: 24 Oct 2019 10:58
URI: http://irep.iium.edu.my/id/eprint/74944

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