Statistical optimization of gelatin immobilisation on modified surface of PCL microcarrier to improve its compatibility

Samsudin, Nurhusna and Hashim, Yumi Zuhanis Has-Yun and Arifin, Mohd Azmir and Mel, Maizirwan and Hamzah, Mohd. Salleh and Nordin, Norshariza and Iis, Sopyan and Jimat, Dzun Noraini (2017) Statistical optimization of gelatin immobilisation on modified surface of PCL microcarrier to improve its compatibility. Jurnal Teknologi, 79 (6). pp. 167-175. E-ISSN 2180-3722

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Abstract

Growing cells on microcarriers may have overcome the limitation of conventional cell culture system. However, the main challenge remains at ensuring the surface biocompatibility with cells. Polycaprolactone (PCL), a biodegradable polymer, has received considerable attention because of its excellent mechanical properties and degradation kinetics that suit various applications, but its non-polar hydrocarbon moiety renders it sub-optimal for cell attachment. In this present study, the aim was to improve biocompatibility of PCL microcarrier by introducing oxygen functional group via ultraviolet irradiation and ozone aeration (UV/O3 system) to allow covalent immobilization of gelatin on the PCL microcarrier surface. Respond surface methodology was used as a statistical approach to optimized parameters that effect the immobilization of gelatin. The parameters used to maximized amount of gelatin immobilize were the mol ratio of COOH:EDAC, NHS concentration and gelatin concentration. The optimum conditions for maximum amount of gelatin (1797.33 µg/g) on the surface of PCL were as follows: 1.5 of COOH:EDAC ratio, 10 mM NHS concentration and, 80 mg/ml gelatin. The result shows that gelatin coated PCL microcarrier promote more and rapid cell adhesion with density of 16.5 ×105 cells/ml as compared to raw PCL microcarrier (2.4×105 cells/ml) and UV/O3 treated PCL microcarrier (4.25×105 cells/ml). Therefore, immobilization of gelatin with optimized parameters onto PCL microcarrier improved biocompatibility of PCL microcarrier. ********************************************************************************************************************************************** Pengkulturan sel menggunakan “microcarriers” mungkin telah mengatasi kekangan sistem kultur sel konvensional. Walau bagaimanapun, cabaran utama untuk memastikan biokompatibiliti (keserasian permukaan) “microcarrier” dengan sel masih belum dapat diatasi sepenuhnya. Polycaprolactone (PCL), sejenis polimer terbiodegradasikan semakin mendapat perhatian berdasarkan ciri-ciri mekanikal yang sangat baik dan kinetik degradasi yang sesuai untuk pelbagai kegunaan. Namun begitu, moieti hidrokarbon tanpa kutub pada PCL menjadikan ia sub-optimum untuk pelekatan sel. Di dalam kajian ini, kumpulan berfungsi oksigen diperkenalkan melalui sistem penyinaran ultraungu dan pengudaraan ozon (system UV/O3) untuk membolehkan imobilisasi (pemegunan) gelatin secara kovalen pada permukaan “microcarrier” PCL dengan menggunakan 1-etil-3-(3- dimetilaminopropil) hidroklorida karbodiimid dan N-hidroksisuksinimid (EDAC/NHS). Keadaan optimum untuk memaksimumkan jumlah gelatin (1797.33 µg/g) pada permukaan PCL adalah seperti berikut: Nisbah1.5 COOH:EDAC, 10 mM NHS dan, 80 mg/ml gelatin.

Item Type:	Article (Journal)
Additional Information:	8648/74717
Uncontrolled Keywords:	Microcarrier, gelatin immobilization, polycaprolactone, statistical optimization Microcarrier, imobilisasi (pemegunan) gelatin, polycaprolactone, pengoptimuman statistik
Subjects:	Q Science > QD Chemistry
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	International Institute for Halal Research and Training (INHART) Kulliyyah of Engineering Kulliyyah of Engineering > Department of Manufacturing and Materials Engineering
Depositing User:	Dr Nurhusna Samsudin
Date Deposited:	23 Oct 2019 09:05
Last Modified:	01 Apr 2024 12:01
URI:	http://irep.iium.edu.my/id/eprint/74717

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