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Microbial hydrolytic enzymes: In silico studies between polar and tropical regions

Abd Latip, Muhammad Asyraf and Abdul Hamid, Azzmer Azzar and Nordin, Noor Faizul Hadry (2019) Microbial hydrolytic enzymes: In silico studies between polar and tropical regions. Polar Science, 20 (part 1). pp. 9-18. ISSN 1873-9652

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Enzyme is important as biocatalyst for industrial and biotechnological applications. Cold active enzymes have showed many advantages compare to mesophilic enzymes. Their cold active and thermolabile characteristics have shown potential benefits in many industries. In silico characterization of hydrolytic enzymes originated from polar and tropical regions was conducted. Amino acid sequences and molecular structures of mesophilic and psychrophilic bacterial enzyme homologues were compared thoroughly. Amino acid sequences from these two homologs do not showed any extraordinary differences. Overall, protein folds were highly similar when psychrophilic homologies were matched with mesophilic homologies. Active site residues located in catalytic domain of both psychrophilic and mesophilic enzymes were highly conserved. Thus, the hydrolytic mechanisms of these cold active enzymes still remain similar to mesophilic enzymes. However, the differences were indicated between these two enzymes at the substrate-binding sites. Cold active enzymes showed unique loop conformations and smaller side chains at the entrances. These characteristics provided larger active site of the enzymes. Larger entrance of active site was expected to stipulate substrates binding and products exiting with lower energy consumption. This part of the enzymes indicated one of the important features of cold active enzyme to work efficiently at lower temperature. Based on this in silico study, cold active enzymes have much more advantages compared to the mesophilic enzymes that made them valuable to be further researched and applied at industrial level.

Item Type: Article (Journal)
Additional Information: 7089/74627 Volume 20, Part 1,: Joint volume of Special Issue on 7th Malaysian International Seminar on Antarctica "MISA7" and Regular Issue Connectivity between Polar and Equatorial climate and Biosphere: from the Poles to the Tropics
Uncontrolled Keywords: Psychrophiles, Cold active enzyme, In silico, Loop
Subjects: Q Science > QR Microbiology
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): International Institute for Halal Research and Training (INHART)
Kulliyyah of Science
Kulliyyah of Science > Department of Biotechnology
Depositing User: AP. Dr. Azzmer Azzar Abdul Hamid
Date Deposited: 07 Oct 2019 15:59
Last Modified: 20 Nov 2019 17:37
URI: http://irep.iium.edu.my/id/eprint/74627

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