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Total phenolic content and antioxidant activity of roselle (Hibiscus sabdariffa) extracts

Haron, Normah and Mat Jusoh, Hanapi and Abul@Abdul Rahman, Syuhaida (2019) Total phenolic content and antioxidant activity of roselle (Hibiscus sabdariffa) extracts. International Journal of Allied Health Sciences, 3 (2). pp. 725-733.

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Introduction: Roselle (Hibiscus sabdariffa) is widely known for its benefits and medicinal properties. The aims of this study were to determine the total phenolic content (TPC) in H. sabdariffa extract, its antioxidant activity and relationship between TPC and its antioxidant scavenging capacity. Methods: The TPC value for each extract of free, bound soluble and bound insoluble phenolic acids was determined by using Folin-Ciocalteu assay. The antioxidant scavenging capacity was evaluated by using DPPH assay. Results: The results obtained in this study showed that bound soluble phenolic acids (7.63 + 0.28 mg GAE/g DW) had the highest TPC value than the free and insoluble phenolic acid extracts (2.78 + 0.20 mg GAE/g DW and 3.61 + 0.85 mg GAE/g DW, respectively) (p<0.05). As for the IC50 values, bound soluble phenolic acid extract exhibited the greatest value(0.62%) as compared to the free and bound insoluble phenolic acid extracts (0.90% and 1.14%, respectively). A positive correlation (R2 = 0.956) between three extracts (free, soluble and insoluble phenolic acids) of TPC and antioxidant radical scavenging capacity (DPPH) was obtained. Conclusion: It can be concluded that phenolic acids in H. sabdariffa extracts have an effect to the antioxidant scavenging capacity particularly in the bound soluble form. This finding proved that H. sabdariffa extracts could be a good source of natural antioxidant for food, cosmetic and pharmaceutical industries.

Item Type: Article (Journal)
Additional Information: 7032/73950
Uncontrolled Keywords: H. sabdariffa, free phenolic, bound phenolic, TPC
Subjects: Q Science > Q Science (General)
Q Science > QD Chemistry
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Science > Department of Biotechnology
Kulliyyah of Allied Health Sciences
Kulliyyah of Allied Health Sciences > Department of Nutrition Sciences
Kulliyyah of Science
Depositing User: Dr. Normah Haron
Date Deposited: 15 Aug 2019 14:29
Last Modified: 15 Aug 2019 14:29
URI: http://irep.iium.edu.my/id/eprint/73950

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