In-vitro cytotoxicity of Trigona itama honey against human lung adenocarcinoma epithelial cell line (A549)

Mohd Salim, Siti Norfitrah and Ramakreshnana, Logaraj and Fong, Chng Saun and Abdul Wahab, Ridhwan and Abdull Rasad, Mohammad Syaiful Bahari (2019) In-vitro cytotoxicity of Trigona itama honey against human lung adenocarcinoma epithelial cell line (A549). European Journal of Integrative Medicine, 30. pp. 1-8. ISSN 1876-3820 E-ISSN 1876-3839

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Abstract

Introduction: Many efforts have been made to identify natural alternatives to reduce the side effects of cytotoxic drugs in cancer treatment. With this in mind, the current study aimed to investigate the cytotoxicity effects of one of the multifloral Malaysian honey, Kelulut honey (Trigona itama), as a potential natural anticancer agent in stimulating apoptosis and cell cycle arrest to a human lung adenocarcinoma epithelial cell line (A549). Methods: The cells were treated with various concentrations of T. itama honey for 24, 48 and 72 hours. The cytotoxicity and cell viability were determined using trypan blue exclusion assay (TBEA) and flow cytometric analysis. Results: The moisture content in the analysed honey was 14.3 ± 0.8%, which was within the accepted international standard. The pH, electrical conductivity and proline content were 3.17 ± 0.02, 0.47 mS/cm - 0.55 mS/cm and 19.1 mg/kg - 20.2 mg/kg respectively. The findings demonstrated a significant dose and time-dependent inhibitory effect of T. itama honey with the maximum cytotoxic effects were observed at 72 hours with 20% concentration of T. itama honey, indicating 100% growth inhibition. Meanwhile, IC50 of T. itama honey treatment for A549 cells was determined as 0.62% v/v. Moreover, T. itama honey has a promising cytotoxic effect and proven capable of inducing cell cycle arrest at G2/M phase at 72 hours of exposure with IC50 concentration. Conclusion: This study provides a prefatory evidence on T. itama honey’s significant anticancer activity against human lung cancer cell lines.

Item Type:	Article (Journal)
Additional Information:	4701/73919
Uncontrolled Keywords:	Anti-cancer; Honey; Human lung adenocarcinoma epithelial cell; In-vitro cytotoxicity; Natural medicine; Trigona itama
Subjects:	R Medicine > R Medicine (General) R Medicine > RA Public aspects of medicine > RA0421 Public health. Hygiene. Preventive Medicine R Medicine > RC Internal medicine R Medicine > RC Internal medicine > RC254 Neoplasms. Tumors. Oncology (including Cancer) R Medicine > RC Internal medicine > RC270 Neoplasms. Tumors. Oncology-Cancer and Other Malignant Neoplasms-Therapeutics T Technology > TX Home economics > TX341 Nutrition. Foods and food supply
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Allied Health Sciences Kulliyyah of Allied Health Sciences > Department of Biomedical Science (Effective:1st July 2011)
Depositing User:	Mrs Siti Norfitrah Mohd Salim
Date Deposited:	11 Sep 2019 15:06
Last Modified:	05 Apr 2020 17:09
URI:	http://irep.iium.edu.my/id/eprint/73919

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