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Determination of cetirizine in human plasma and validation method using HPLC technique

Zuhri, Siti Hazar and Azad, Abul Kalam and Mia, Abdur Rashid and Helaluddin, Abul Bashar Mohammed and Mohd. Nawi, Mohamed Sufian (2019) Determination of cetirizine in human plasma and validation method using HPLC technique. Journal of Drug Delivery and Therapeutics, 9 (2-S (March-April)). pp. 243-247. ISSN 2250-1177

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Abstract

This reseach project is dedicated to analyze cetirizine in human plasma by using HPLC qualitatively and quantitatively, to make a reliable bioanalysis of this drug in human plasma with a goal to utilise it in pharmacokinetics studies. In this research project, cetirizine was extracted from plasma using liquid-liquid extraction by dichloromethane and ethyl acetate. The results in terms of accuracy and precision are compared. For both of the methods, amlodipine was used as the internal standard. Separation was carried out by Phenomenex C18 chromato graphy column. The mobile phase used here is 35:65, v/v of Acetonitrile to 0.3% triethylamine (TEA) buffer fixed at pH 3 by phosphoric acid, with flow rate of 1mL/min. The column temperature was set at 30 °C. Variable wavelength detector (VWD) was used, and detection was set at 237 nm. The analysis was linear from 30 ng/mL to 500 ng/mL. Overall the recovery is more than 90% when using dichloromethane and more than 65% when using ethyl acetate.

Item Type: Article (Journal)
Additional Information: 4478/72827
Uncontrolled Keywords: Bioanalysis, Method validation, Cetirizine, Human plasma, HPLC
Subjects: Q Science > QD Chemistry
R Medicine > RM Therapeutics. Pharmacology
R Medicine > RS Pharmacy and materia medica
R Medicine > RS Pharmacy and materia medica > RS403 Materia Medica-Pharmaceutical Chemistry
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Pharmacy > Department of Pharmaceutical Chemistry
Kulliyyah of Pharmacy
Depositing User: Dr. A. B. M. Helal Uddin
Date Deposited: 26 Jun 2019 09:59
Last Modified: 26 Jun 2019 09:59
URI: http://irep.iium.edu.my/id/eprint/72827

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