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Development and validation of UV-vis spectroscopic method of assay of carbamazepine in microparticles

Mawazi, Saeid Mezail and Ab. Hadi, Hazrina and Abdullah, Sinan Mohamed and Doolanea, Abd Almonem (2019) Development and validation of UV-vis spectroscopic method of assay of carbamazepine in microparticles. International Journal of Applied Pharmaceutics, 11 (1). pp. 34-37. ISSN 0975-7058

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Abstract

Objective: This study aimed to develop a new, rapid, robust, effective, inexpensive, and accurate UV-Vis method for the quantification analysis of carbamazepine (CBZ) in the carbamazepine-loaded microparticles. Methods: CBZ was encapsulated in ethyl cellulose microparticles by a solvent evaporation method using polyvinyl alcohol (PVA) as a stabilizer. Methanol was used to dissolve CBZ followed by dilution with distilled water as diluent. CBZ drug, excipients, and microparticles were subjected to specificity, solution stability, linearity, precision and accuracy to confirm and ensure the validity of this method. Results: The results showed no interference from the excipients in the selected wavelength 286 nm. It was exhibited linearity in the range 2-12 μg/ml with R 2 = 0.9992. CBZ solution was stable during 24 h. Accuracy and precision were within the accepted limits (100±2%). All results were in accordance to the ICH-Q2 guideline. Conclusion: As a conclusion, CBZ could be quantified from loaded EC microparticles using UV-Vis spectrophotometer at 286 nm. Therefore, this method can be used for the quantification analysis of CBZ in CBZ-loaded microparticles can be utilized also as an alternative method to calculate CBZ in different dosage forms. © 2019 The Authors.

Item Type: Article (Journal)
Additional Information: 5589/72710
Uncontrolled Keywords: Carbamazepine, Microparticles, Method validation, UV-Vis, Spectrophotometer
Subjects: R Medicine > RS Pharmacy and materia medica
R Medicine > RS Pharmacy and materia medica > RS192 Materia Medica-Pharmaceutical Technology
Kulliyyahs/Centres/Divisions/Institutes: Kulliyyah of Nursing
Kulliyyah of Nursing > Department of Basic Medical Sciences for Nursing
Kulliyyah of Pharmacy
Kulliyyah of Pharmacy > Department of Pharmaceutical Technology
Depositing User: Dr Hazrina Ab Hadi
Date Deposited: 18 Jun 2019 16:35
Last Modified: 18 Jun 2019 16:39
URI: http://irep.iium.edu.my/id/eprint/72710

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