Optimization of hyperglycemic induction in zebrafish and evaluation of its blood glucose level and metabolite fingerprint treated with psychotria malayana Jack Leaf extract

Benchoula, Khaled and Khatib, Alfi and Quzwain, Fairuz M. C. and Che Mohamad, Che Anuar and Wan Sulaiman, Wan Mohd Azizi and Abdul Wahab, Ridhwan and Ahmed, Qamar Uddin and Abdul Ghaffar, Majid and Saiman, Mohd Zuwairi and Alajmi, Mohamed Fahad and El-Seedi, Hesham (2019) Optimization of hyperglycemic induction in zebrafish and evaluation of its blood glucose level and metabolite fingerprint treated with psychotria malayana Jack Leaf extract. Molecules, 24 (8). pp. 1-22. ISSN 1420-3049

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Abstract

A standard protocol to develop type 1 diabetes in zebrafish is still uncertain due to unpredictable factors. In this study, an optimized protocol was developed and used to evaluate the anti-diabetic activity of Psychotria malayana leaf. The aims of this study were to develop a type 1 diabetic adult zebrafish model and to evaluate the anti-diabetic activity of the plant extract on the developed model. The ability of streptozotocin and alloxan at a different dose to elevate the blood glucose levels in zebrafish was evaluated. While the anti-diabetic activity of P. malayana aqueous extract was evaluated through analysis of blood glucose and LC-MS analysis fingerprinting. The results indicated that a single intraperitoneal injection of 300 mg/kg alloxan was the optimal dose to elevate the fasting blood glucose in zebrafish. Furthermore, the plant extract at 1, 2, and 3 g/kg significantly reduced blood glucose levels in the diabetic zebrafish. In addition, LC-MS-based fingerprinting indicated that 3 g/kg plant extract more effective than other doses. Phytosterols, sugar alcohols, sugar acid, free fatty acids, cyclitols, phenolics, and alkaloid were detected in the extract using GC-MS. In conclusion, P. malayana leaf aqueous extract showed anti-diabetic activity on the developed type 1 diabetic zebrafish model.

Item Type:	Article (Journal)
Additional Information:	6915/72563
Uncontrolled Keywords:	Type 1 diabetes; zebrafish; Psychotria malayana; alloxan; insulin; streptozotocin
Subjects:	R Medicine > RS Pharmacy and materia medica
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Allied Health Sciences Kulliyyah of Allied Health Sciences > Department of Biomedical Science (Effective:1st July 2011) Kulliyyah of Pharmacy Kulliyyah of Pharmacy > Department of Basic Medical Sciences Kulliyyah of Pharmacy > Department of Pharmaceutical Chemistry
Depositing User:	Dr. Alfi Khatib
Date Deposited:	12 Jun 2019 12:34
Last Modified:	31 Jul 2019 15:12
URI:	http://irep.iium.edu.my/id/eprint/72563

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