Gene expression of selected apoptotic markers in Human Oral Carcinoma (HSC-3) cell lines trewated with myrmecodia pendans plant extract

Iskandar, Siti Hajar and Wan Yusry, Wan Nur Aisyah and Lestari, Widya and Arief Ichwan, Solachuddin Jauhari (2019) Gene expression of selected apoptotic markers in Human Oral Carcinoma (HSC-3) cell lines trewated with myrmecodia pendans plant extract. In: 8th Dental Students’ Scientific Conference IIUM 2019, 25th February 2019, Kuantan, Pahang. (Unpublished)

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Abstract

Introduction: Myrmecodia pendans (M. pendans), also known as Sarang Semut is an epiphyte with potential as a new therapeutic agent. Recently, we reported that M. pendans induced apoptotic activity in human oral squamous carcinoma (HSC-3) cell line. This study aimed to study how M. pendans extract affected apoptotic markers BAX/BCL-2 expression. Materials and methods: M. pendans was purchased from West Papua, Indonesia. The hypocotyl was allowed to dry thoroughly, then extracted aqueously. The extract was kept at -80 °C before freeze drying. The crude extract was used in the experiments. HSC-3 cells were cultured in Dulbecco’s Modified Essential Medium, then exposed to M. pendans at 2.5, 5.0 and 7.5 mg/ml for 24 and 48 h. Doxorubicin (0.005 mg/ml) was used as positive control. BAX and BCL-2 expression was analysed by Quantitative Real Time Polymerase Chain Reaction. Results: BAX and BCL-2 expression in HSC-3 cells was affected by M. pendans. At 24 h, highest levels of BAX and BCL-2 were observed with M. pendans at 2.5 mg/ml, while downregulation was observed at 5 mg/ml. However, after 48 h, BAX and BCL-2 were downregulated at all concentrations. The ratio of BAX/ BCL-2 in HSC-3 cells at 24 h showed significant upregulation at all treatment concentrations, but was downregulated after 48 h. Conclusion: M. pendans extract induced apoptotic activity in HSC-3 cells. This study suggests that induction of apoptosis in HSC-3 cell line is regulated via the pro-apoptotic (BAX) and anti- apoptotic (BCL -2) pathways.

Item Type:	Conference or Workshop Item (Speech/Talk)
Additional Information:	6863/71884
Uncontrolled Keywords:	M. pendans, HSC-3, apoptotic, BAX, BCL-2
Subjects:	R Medicine > RK Dentistry
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Dentistry Kulliyyah of Dentistry > Department of Fundamental Dental and Medical Sciences Kulliyyah of Dentistry > Department of Oral Maxillofacial Surgery and Oral Diagnosis
Depositing User:	Dr Widya Lestari
Date Deposited:	02 May 2019 14:41
Last Modified:	02 May 2019 14:43
URI:	http://irep.iium.edu.my/id/eprint/71884

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