Development of solid state fermentation for protease enzyme production from the Indah Water Konsortium sludge cake

Karim, Tanzeed and Alam, Md. Zahangir (2018) Development of solid state fermentation for protease enzyme production from the Indah Water Konsortium sludge cake. In: 5th International Conference on Biotechnology Engineering (ICBioE 2018), 19th-20th September 2018, Kuala Lumpur.

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Abstract

The purpose of this research was to tackle the increase amount of sludge being produced every year from wastewater treatment plant and to figure out an alternative form of way to produce commercial protease enzyme. The wastewater sludgecake from Indah Water Konsortium Sdn Bhd was used as the substrate media and a potential low-cost media for protease enzyme production. For the design of the experiments, eleven parameters were screened and the three most significant parameters were found as glucose, tween-80 and moisture content which were further optimized using Face Centered Central Composite Design to find the optimum condition for the production. The optimization study showed that 4% glucose(w/v), 2% tween-80(v/v) and 40% moisture Content(w/v) produces the highest protease activity of 92.141 U/ml. The analysis of variance (ANOVA) also showed that the optimization model was significant with p < 0.0038 and R 2 0.8526.

Item Type:	Conference or Workshop Item (Plenary Papers)
Additional Information:	4157/70583
Uncontrolled Keywords:	protease; sewage sludge; bacteria; Plackett-Burman; Face Centered Central Composite Design
Subjects:	T Technology > TP Chemical technology > TP155 Chemical engineering
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button):	Kulliyyah of Engineering > Department of Biotechnology Engineering Kulliyyah of Engineering
Depositing User:	Dr. Md. Zahangir Alam
Date Deposited:	28 Feb 2019 09:19
Last Modified:	28 Feb 2019 09:19
URI:	http://irep.iium.edu.my/id/eprint/70583

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