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In silico structural and functional annotation of nine essential hypothetical proteins from Streptococcus pneumoniae

Razali, Khairiah and Abdul Hamid, Azzmer Azzar and Md Zin, Noor Hasniza and Mohammad Bunnori, Noraslinda and Ahmad Yusof @Hanafi, Hanani and Kamarudin, Kamarul Rahim and Mohammed Rehan, Aisyah (2019) In silico structural and functional annotation of nine essential hypothetical proteins from Streptococcus pneumoniae. Indonesian Journal of Chemistry. ISSN 1411-9420 E-ISSN 2460-1578 (In Press)

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The ability of Streptococcus pneumoniae to induce infections relies on its virulence factor machinery. A previous CRISPR interference (CRISPRi) study had identified 254 essential proteins that may be responsible for the pathogenicity of S. pneumoniae serotype 2 strain D39. However, 39 of them were functionally and structurally uncharacterized. Hence, by using in silico approach, this study aimed to annotate the function and structure of these un-annotated proteins. Initially, all 39 proteins went through primary screening for template availability and pathogenicity. From there, 11 of them were selected and underwent further physicochemical, functional, and structural categorization through an integrated bioinformatics approach by means of amino acid sequence- and structure- based analyses. The obtained data revealed that 9 targeted proteins showed a high possibility to be involved in either cell viability or cell pathogenicity mechanism of the bacterium, with SPD_1333 and SPD_1743 being the two most promising proteins to be further studied. Findings from this study can help in facilitating a better understanding of pathogenic ability of this microorganism and enhance drug development and target identification processes in the aim of improving pneumococcal disease control.

Item Type: Article (Journal)
Additional Information: 7089/70283
Uncontrolled Keywords: hypothetical proteins; S. pneumoniae strain D39; in silico analysis of protein; bioinformatics tools
Subjects: Q Science > Q Science (General)
Kulliyyahs/Centres/Divisions/Institutes (Can select more than one option. Press CONTROL button): Kulliyyah of Allied Health Sciences
Kulliyyah of Allied Health Sciences > Department of Biomedical Science (Effective:1st July 2011)
Kulliyyah of Science
Kulliyyah of Science > Department of Biotechnology
Depositing User: Dr Noor Hasniza Md Zin
Date Deposited: 10 Dec 2019 11:48
Last Modified: 10 Dec 2019 11:48
URI: http://irep.iium.edu.my/id/eprint/70283

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